Culturing, transfection and NBD-labeled lipid uptake assay in HeLa-CDC50A-OE cells

Research Community
Research Community

ASAP supports three programs and serves as a member of multiple consortia to advance research for the benefit of the global Parkinson’s disease community.
Explore
Highlights
Highlights

ASAP shares the impact that our initiative, network, and supported programs have had on providing new insights into Parkinson’s disease. Discover news, interviews, awards, and annual impact.
Explore
Open Science
Open Science

ASAP is dedicated to facilitating a research environment where meaningful collaboration, research-enabling resources, and data sharing provides the answers we need to understand, diagnose, and treat Parkinson’s disease. Discover our open science philosophy.
Explore
Tools & Resources
Tools & Resources

ASAP is committed to developing and openly sharing research outputs, including data, code, protocols, and key lab materials, to help the scientific community build upon existing work.
Explore

Description

The NBD‑labeled lipid uptake assay outlines the procedures for seeding, transient transfection, and lipid uptake measurement in HeLa‑CDC50A‑OE cells, monitored by flow cytometry. This assay enables transient overexpression of flippase constructs and allows quantitative assessment of their NBD‑lipid uptake activity within a cellular environment.

Identifier (DOI)

10.17504/protocols.io.bp2l6zo8kgqe/v1

Discovery Team

Team Vangheluwe

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Klara Scholtissek

External Collaborator
Filip Pamula

External Collaborator

Culturing, transfection and NBD-labeled lipid uptake assay in HeLa-CDC50A-OE cells

Output Details

Meet the Authors

Klara Scholtissek

Filip Pamula

Related Research

Flow cytometry data related to ‘The lipid flippase ATP10B enables cellular lipid uptake under stress conditions’

Flow cytometry data from the manuscript “Fluorescence-Gated Flow Cytometry Approach for Measuring Lipid Flippase Activity in Mamma-lian Cells”

Spermidine suppresses glial inflammation and parkinsonian abnormalities in ATP13A2 deficiency