In vivo carbon fiber electrode thread (CFET) implantation and testing procedures
By Emma Sherrell onProcedures for implanting and testing CFET devices in rats are described.
In vitro FSCV testing of carbon fiber electrodes to characterize functional operation in dopamine detection
By Emma Sherrell onMethods to measure the performance characteristics of carbon fiber electrodes for neurochemical recording.
Immunohistochemistry for carbon fiber thread electrodes
By Emma Sherrell onMethods for immunofluorescent staining of brain tissue with indwelling electrodes.
Immunofluorescent staining for neuronal marker MAP2
By Emma Sherrell onThis is the protocol for immunofluorescent staining for neuronal marker MAP2.
Human neuroblastoma cell line SH-SY5Y culturing
By Emma Sherrell onThis is the brief protocol for seeding and culturing human neuroblastoma SH-SY5Y cells.
Histology and retention of implanted carbon fiber thread electrodes in fixed tissue
By Emma Sherrell onMethods to cut and retain implanted carbon fiber electrodes in brain tissue.
HEK293T SNCA-A53T-mRuby3 SNCA-A53T-mClover3
By Emma Sherrell onCell Line: Transfected with two plasmids encoding mRuby3- and mClover3-tagged SNCA p.Ala53Thr expressed under the control of CMV promoters.
HEK293E_Ecto short VLDLR-C-tag
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing the ectodomain of VLDLR fused to a C-terminal C-tag (EPEA) under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu WT
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu TL4
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu TL3
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu TL2+3
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu TL1
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu DD3
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
Dynamic behaviour restructuring mediates dopamine-dependent credit assignment
By Julia Julia Leonard onSource data for Nature manuscript: Dynamic behavior restructuring mediates dopamine-dependent credit assignment.
Y-Maze protocol
By Julia Julia Leonard onThe Y-maze is a quick and easy way to evaluate a rodent’s spatial working memory by measuring its ability to effectively explore a Y-shape maze.
Protocol for α-synuclein (αSyn) proximity ligation assay (PLA) for detecting α-synuclein oligomers in rodents
By Julia Julia Leonard onProtocol for α-synuclein (αSyn) proximity ligation assay (PLA) for detecting α-synuclein oligomers in rodents.
Immunostaining
By Julia Julia Leonard onThis is a protocol that describes how to use antibody staining to detect cellular epitopes by immunofluorescence microscopy.
Ubiquitin immunoprecipitation using an anti-ubiquitin nanobody
By Julia Julia Leonard onThis protocol describes an immunoprecipitation method to detect ubiquitination on a protein of interest.
HEK293E_Clu DD1
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.