Purification of SRC kinase
By savannah onThis protocol describes how to express and purify SRC kinase.
In vivo microdialysis for striatal DA release
By Emma Sherrell onTo assess local effects of nomifensine (DAT and NET inhibitor) on striatal DA release in microdialysis experiments.
Brain clarification for neuromelanin visualisation by OPT
By Emma Sherrell onProtocol for the clarification of whole mouse brains to allow neuromelanin visualisation by Optical Projection Tomography.
Purification of FIP200 CTR-GFP
By savannah onThis protocol describes how to express and purify GFP-FIP200 (CTR).
Open field using Ethovision
By Emma Sherrell onProtocol for general locomotion in rodents using open field arenas and Ethovision video tracking. Purpose: Assess cognitive, spontaneous motor activity, and anxiety. Note that there are many variations of this test. The authors perform this test during the mouse's dark cycle (i.e. their active phase).
Modelling human neuronal catecholaminergic pigmentation in rodents recapitulates age-related multisystem neurodegenerative deficits
By Emma Sherrell onModels for neurodegenerative diseases lack of NM, a pigment found in human brains. A new transgenic mouse model, mimics human distribution and shows age-related neuronal dysfunction and degeneration, offering potential for research in brain aging.
Functional efficacy of the MAO-B inhibitor safinamide in murine substantia nigra pars compacta dopaminergic neurons in vitro: a comparative study with tranylcypromine
By Emma Sherrell onSafinamide (SAF) is used for PD by enhancing dopamine signal. SAF prolongs recovery from dopamine-mediated firing inhibition in SNpc DAergic neurons, mildly compared to tranylcypromine, suggesting multiple sites of action for SAF's therapeutics.
Protocol for quantification of neurons expressing alpha-synuclein in the non-human primate brain
By Emma Sherrell onHere, the authors describe a method based on an AI deep learning algorithm (Aiforia) for the purpose of accurately quantifying the number and location of neurons expressing alpha-synuclein throughout pre-defined regions of interest (cortical and sub-cortical locations) in the non-human primate brain.
