Copy of RNA extraction from mouse tissue
By onProtocol used in the Gradinaru lab for RNA extraction from mouse tissue
Genetically encoded self-assembling synuclein for tunable Parkinsonian pathology in vitro and in vivo
By onThis paper describes the self-assembling synuclein (SAS) system, a genetically-encoded tool for inducing temporally-controllable, tunable PD pathology in a wide range of in vitro and in vitro models.
Transfection of HEK cells with Lipofectamine 3000
By onProtocol used in the Gradinaru lab to transfect HEK cells with lipofectamine 3000
Western Blot with iBind System
By onProtocol used in the Gradinaru lab for western blot using the iBind Automated Western System
Primary Neuron Extraction
By onProtocol used in the Gradinaru lab to extract primary neurons from mouse on day E17
Mouse Open Field
By onProtocol used in the Gradinaru lab for general locomotion in rodents using open field arenas and Ethovision video tracking
sPAtial Neuronal Gene Expression Atlas (PANGEA) Application
By onPANGEA is a web app for visualizing brain regional gene expression data. It's a spatial atlas showing gene patterns related to neurons vulnerable to α-synuclein pathology.
Immunocytochemistry (Henderson Lab)
By onProtocol for immunocytochemistry on mammalian cells in 24 and 96 well plates.
Network analysis of α-synuclein pathology progression reveals p21-activated kinases as regulators of vulnerability
By onMisfolded α-synuclein accumulation in PD leads to pathogenic processes. We mapped α-synuclein pathology in mice to identify vulnerable regions and potential therapeutic targets, highlighting group II PAK inhibitors as promising for treating PD.
BugSigDB – Parkinson’s Disease
By onA Comprehensive Database of Published Microbial Signatures in PD
Histology Fushiki et al 2024
By onHistology protocol for Fushiki et al 2024 , to quantify Th+ cells and visualize the dorsal-tier or ventral-tier of SNc DANs.
Severe GBA1 variants drive the GBA-PD clinical phenotype: implications for counselling and clinical trials
By onVariants in the GBA1 gene are common genetic risk factors for Parkinson's disease. A study compared clinical phenotypes in GBA-PD and idiopathic PD patients, finding that only severe GBA-PD cases show a more severe clinical profile.
Exploring the relationship between GBA1 host genotype and gut microbiome in the GBA1L444P/WT mouse model: Implications for Parkinson disease pathogenesis
By onHeterozygous *GBA1* variants are common in Parkinson's disease. This study found no significant impact of the *GBA1* L444P variant on gut microbiome composition in mice, suggesting other factors may contribute to disease penetrance.
Open Field & Cylinder test Behavior Assays
By onProtocols for mouse behavioral studies using the open field test and the cylinder test from Fushiki et al 2024.
Single-cell RNA-sequencing data from A Vulnerable Subtype of Dopaminergic Neurons Drives Early Motor Deficits in Parkinson’s Disease
By onSingle-nucleus RNA profiling of the ventral midbrain of MitoPark mice, their littermate controls and C57BL/6 mice at 8, 16, and 24 weeks of age (8 mice in each group; 4 males and 4 females).
Non-human Primate Arm-reaching Behavioral Tasks
By onProtocol for training and testing non-human primates in arm-reaching tasks to evaluate parkinsonian animals
Specific cell states underlie complex tissue regeneration in spiny mice
By onThis paper compares regenerative and fibrotic healing to identify injury-induced cell states associated with persistent cell cycle progression and complex tissue regeneration in mammals.
Key Resource Table for Segregated basal ganglia output pathways correspond to genetically divergent neuronal subclasses
By onComprehensive key resource table for the publication: "Segregated basal ganglia output pathways correspond to genetically divergent neuronal subclasses", Mendelsohn et al 2025.
