Movement-related activity in the internal globus pallidus of the Parkinsonian macaque
By savannah onThis study of MPTP-induced Parkinsonism in macaques indicates that changes in the timing of task-related neural activity of the internal segment of the globus pallidus (GPi) correlate with the severity of motor impairments.
Dissociation of putative open loop circuit from ventral putamen to motor cortical areas in humans I: High-resolution connectomics
By savannah onHigh-resolution connectomics in human participants provides evidence for a non-canonical circuit linking the limbic ventral putamen to the motor cortex. The data is in line with previous findings in nonhuman primates.
Human Postmortem-Derived Brain Sequencing Collection (Harmonized Collection)
By Emma Sherrell onThe Human Postmortem-Derived Brain Sequencing Collection is a harmonized repository comprised of sequencing data contributed by ASAP CRN teams.
Temporal landscape of mitochondrial proteostasis governed by the UPRmt_PRIDE deposition
By savannah onMass spectrometry proteomics data associated with "Temporal landscape of mitochondrial proteostasis governed by the UPRmt" deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD038403.
Nuclei counting after Trypan Blue staining
By savannah onNuclei counting after Trypan Blue staining.
Nextflow pipeline for Nanopore WGS analysis
By Emma Sherrell onNextflow pipeline to process whole genome long-read sequencing data generated in the context of ASAP project.
Protein structure prediction with AlphaFold-Multimer
By savannah onThis protocol describes the protein structure prediction with AlphaFold-Multimer.
Functional efficacy of the MAO-B inhibitor safinamide in murine substantia nigra pars compacta dopaminergic neurons in vitro: A comparative study with tranylcypromine
By Emma Sherrell onSafinamide (SAF) is used for PD by enhancing dopamine signal. SAF prolongs recovery from dopamine-mediated firing inhibition in SNpc DAergic neurons, mildly compared to tranylcypromine, suggesting multiple sites of action for SAF's therapeutics.
CREsted (Cis-Regulatory Element Sequence Training, Explanation and Design): A deep learning package for training enhancer models on single-cell ATAC sequencing (scATAC-seq) data
By Emma Sherrell onCREsted offers detailed analyses and tutorials for studying enhancer codes and creating synthetic enhancer sequences at cell type-specific, nucleotide-level resolution.
Kinase activity assays Src and CK2
By savannah onThis protocol details about the Kinase activity assays of Src and CK2.
snRNA data from Gaertner & Oram et al., 2024
By Emma Sherrell onDAT-Cre mice with a nuclear GFP reporter and either WT or heterozygous G2019S mutant alleles for the gene Lrrk2. Samples contain tissue from midbrains after isolation of DAT+ nuclei via microfluidic sorting for GFP.
KOLF2.1J DJ1 L166P 2
By Emma Sherrell onThe iPSC lines used for the generation of the DJ1 L166P point mutation were from KOLF 2.1 J background (Pantazis et al., 2021).
Purification mCherry-ATG13 IDR
By savannah onThis protocol describes how to express and purify mCherry-ATG13 IDR.
Purification GFP-ATG13 IDR
By savannah onThis protocol describes how to express and purify GFP-ATG13 IDR.
Datasets and key resources table used in Nebuloni, F. et al. (2024) “A fluid-walled microfluidic platform for human neuron microcircuits and directed axotomy”
By savannah onThe files found here are associated to Nebuloni, F. et al. (2024) study and contains: (a) all source and supplementary data and (b) key resources table.
Luminex-based multiplex cytokine analysis protocol used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By savannah onLuminex-based multiplex cytokine analysis protocol used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration."
KOLF2.1J DJ1 L166P 1
By Emma Sherrell onThe iPSC lines used for the generation of the DJ1 L166P point mutation were from KOLF 2.1 J background (Pantazis et al., 2021).
