Thawing of mouse embryonic fibroblasts (MEFs) for hPSC cultures
By savannah onThis protocol describes the thawing of mouse embryonic fibroblasts (MEFs) as feeder cells for human pluripotent stem cell (hPSC) culture.
Thawing of hPSCs grown on MEFs
By savannah onThis protocol describes the standard procedure of thawing human pluripotent stem cells (hPSCs) on inactivated mouse embryonic fibroblasts (MEFs).
Thawing of feeder-free hPSCs
By savannah onThis protocol describes the procedure of thawing feeder-free human pluripotent stem cells (hPSCs) using mTeSR-plus or StemFlex.
Tet-off TauRD (P301L/V337M)
By savannah onTet-off TauRD (P301L/V337M). Associated with the following preprint: Saha et al., 2022 (published on biorxiv on February 19, 2022). https://www.biorxiv.org/content/10.1101/2022.02.18.481043v1.full
Tet-off TauRD (HEK293T)
By savannah onAssociated with the following preprint: Saha et al., 2022 (published on biorxiv on February 19, 2022). https://www.biorxiv.org/content/10.1101/2022.02.18.481043v1.full
Tet-off Full Length Tau (0N4R, P301L/V337M)
By savannah onTet-off Full Length Tau (0N4R, P301L/V337M). Associated with the following preprint: Saha et al., 2022 (published on biorxiv on February 19, 2022). https://www.biorxiv.org/content/10.1101/2022.02.18.481043v1.full
Tet-off FLTau (HEK293T)
By savannah onAssociated with the following preprint: Saha et al., 2022 (published on biorxiv on February 19, 2022). https://www.biorxiv.org/content/10.1101/2022.02.18.481043v1.full
Targeted neuronal activation of the gastrointestinal tract shapes the environment of the gut in mice
By savannah onThe enteric nervous system (ENS) integrates cues from the brain and from local signals in the gut to coordinate responses that shape the intestinal milieu. Here, the authors examine the role of neurons in modulating intestinal physiology, mucosal immunity, and gut microbiome structure.
Subcloning of genotype-confirmed hPSCs clones
By savannah onThis protocol describes a standard procedure for subcloning of genotype-confirmed human pluripotent stem cells (hPSCs).
Structure of wild-type PPM1H phosphatase at 3.1 Angstrom resolution
By savannah onStructure of wild-type PPM1H phosphatase at 3.1 Angstrom resolution (as reported in 10.15252/embr.202152675) deposited in the Protein Data Bank with code 7KPR.
Structure of PPM1H phosphatase with manganese ions at the active site
By savannah onStructure of PPM1H phosphatase with manganese ions at the active site (as reported in 10.15252/embr.202152675) deposited in the Protein Data Bank with code 7N0Z.
Standard operating procedure: Mouse transcardiac perfusion protocol
By savannah onMouse transcardiac perfusion protocol and storage.
Standard operating procedure: Mouse stereotaxic intracranial injection surgery
By savannah onThe intent of this standard operating procedure (SOP) is to describe procedures for mouse stereotaxic intracranial injection surgery.
Standard operating procedure for the isolation of genetically engineered hPSCs clones in a high-throughput way
By savannah onThis collection describes a standard procedure for isolating single human pluripotent stem cell (hPSC) clones in a high-throughput way. This collection follows nucleofection of hPSCs as described in detail in the collection “Nucleofection (Amaxa) and electroporation (Biorad) of hPSCs.” dx.doi.org/10.17504/protocols.io.b4qnqvve
Single cell survival assay
By savannah onThis protocol describes the experimental procedure used to measure single cell survival rates post nucleofection of human pluripotent stem cells (hPSCs).
Seeding nucleofected hPSCs in 96-well plates using limited dilution
By savannah onThis protocol describes a standard procedure for seeding nucleofected human pluripotent stem cells (hPSCs) in 96-well plates using limited dilution. This protocol follows nucleofection of hPSCs as described in detail in the collection “Nucleofection (Amaxa) and electroporation (Biorad) of hPSCs.” dx.doi.org/10.17504/protocols.io.b4qnqvve
Sample preparation for aCGH karyotyping
By savannah onThis protocol describes the standard procedure preparing cell pellets from human pluripotent stem cells (hPSCs) cultured on MEFs for outsourced aCGH karyotyping.
Preparing samples for NGS
By savannah onThis protocol describes a standard procedure used to prepare PCR samples for Next Generation Sequencing (NGS).
Preparing plasmids for nucleofection of hPSCs
By savannah onThis protocol describes the standard procedure for the preparation of plasmids to be delivered into human pluripotent stem cells (hPSCs) using nucleofection.
Preparing of genomic DNA from in vitro cultured cells
By savannah onThis protocol describes a standard procedure for preparing crude cell lysate which can be further analyzed by PCR.
