Kufor-Rakeb Syndrome-Associated Psychosis: A Novel Loss-of-Function ATP13A2 Variant and Response to Treatment
By onATP13A2 mutations cause Kufor-Rakeb syndrome, leading to parkinsonism with various neurological symptoms. Quetiapine showed promise in treating associated psychosis with good tolerance, suggesting its potential for managing such cases effectively.
Colonic Migrating Motor Complexes
By onProtocol for ex vivo assay of colonic migrating motor complexes (CMMCs) in mouse colons.
Quantification of area and optical density of intracellular neuromelanin with Image J
By onQuantification of area and optical density of intracellular neuromelanin with Image J.
Source data for “Feed-forward metabotropic signaling by Cav1 Ca2+ channels supports pacemaking in pedunculopontine cholinergic neurons”
By onSource Data and images from the paper: Feed-forward metabotropic signaling by Cav1 Ca2+ channels supports pacemaking in pedunculopontine cholinergic neurons
Quantification of area and optical density of intracellular neuromelanin with TruAI in H&E stained sections
By onQuantification of area and optical density of intracellular neuromelanin with TruAI in H&E stained sections.
Protocol for preparing post-mortem tissue for intracellular neuromelanin quantification in H&E-stained sections
By onProtocol for preparing post-mortem tissue for intracellular neuromelanin quantification in H&E-stained sections.
Protocol for α-synuclein (αSyn) proximity ligation assay (PLA) for detecting α-synuclein oligomers in rodents
By onProtocol for α-synuclein (αSyn) proximity ligation assay (PLA) for detecting α-synuclein oligomers in rodents.
Y-Maze Protocol
By onThe Y maze assesses rodents' spatial working memory by observing their tendency to explore new arms over revisiting old ones. Rodents with intact memory will alternate between arms before revisiting.
Dynamic behaviour restructuring mediates dopamine-dependent credit assignment Creators
By onSource Data for Nature manuscript: Dynamic behaviour restructuring mediates dopamine-dependent credit assignment
Immunostaining
By onThis is a protocol that describes how to use antibody staining to detect cellular epitopes by immunofluorescence microscopy.
HEK293T SNCA-A53T-mRuby3 SNCA-A53T-mClover3
By onTransfected with two plasmids encoding mRuby3- and mClover3-tagged SNCA p.Ala53Thr expressed under the control of CMV promoters.
Induction of aggregation in alpha-synuclein-expressing cells by treatment with preformed fibrils (PFFs)
By onThis protocol details how to efficiently produce alpha-synuclein aggregates in cells by templating the misfolding of intracellular alpha-synuclein through treatment with preformed fibrils of alpha-synuclein (PFFs).
Ubiquitin immunoprecipitation using an anti-ubiquitin nanobody
By onThis protocol describes a method to detect ubiquitination on a protein of interest. This technique relies on immunoprecipitation (IP) of ubiquitinated proteins from a cell lysate through the use of an anti-ubiquitin nanobody coupled to agarose beads. The eluate can be run on SDS-PAGE to determine whether the protein of interest was recovered in the IP and, therefore, ubiquitinated.
HEK293T TetOff-SNCA-A53T mRuby3-Galectin-3 mClover3-Galectin-3
By onID HEK293T TetOff-SNCA-A53T mRuby3-Galectin-3 mClover3-Galectin-3 AC CVCL_D4BX SY Syn10 Galectin-3 FRET CC Characteristics: Transduced with an all-in-one TetOff construct for the controllable expression of untagged SNCA p.Ala53Thr (from parent cell line). CC Characteristics: Additional expression of two plasmids encoding mRuby3- and mClover3-tagged LGALS3, enabling a FRET-based readout of endolysosome damage (Direct_author_submission). CC Transfected with: HGNC; 11138; SNCA (with p.Ala53Thr). CC Transfected with: HGNC; 6563; LGALS3. CC Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. CC Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). CC Transfected with: UniProtKB; Q8ISF8; Entacmaea quadricolor eqFP611 (modified to produce the bright RFP mRuby3). CC Transfected with: UniProtKB; P42212; GFP (with p.Ser30Arg, p.Tyr39Asn, p.Ser65Gly, p.Gln69Ala, p.Phe99Ser, p.Asn105Thr, p.Tyr145Phe, p.Asn149Tyr, p.Met153Thr, p.Gly160Cys, p.Val163Ala, p.Ile171Val, p.Thr203His, p.Ala206Lys = mClover3). CC Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd). CC Transfected with: UniProtKB; P00552; Transposon Tn5 neo. CC Transformant: NCBI_TaxID; 28285; Adenovirus 5. CC Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) . CC Miscellaneous: Cell line information from personal communication of Yuste-Checa P. CC Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. OX NCBI_TaxID=9606; ! Homo sapiens (Human) hi CVCL_D4BW ! HEK293T TetOff-SNCA-A53T SX Female AG Fetus CA Transformed cell line Parental cell line: HEK293T (RRID:CVCL_0063)
HEK293T TetOff-SNCA-A53T
By onID HEK293T TetOff-SNCA-A53T AC CVCL_D4BW SY Syn10 CC Characteristics: Transduced with an all-in-one TetOff construct for the controllable expression of untagged SNCA p.Ala53Thr (Direct_author_submission). CC Transfected with: HGNC; 11138; SNCA (with p.Ala53Thr). CC Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. CC Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). CC Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd). CC Transfected with: UniProtKB; P00552; Transposon Tn5 neo. CC Transformant: NCBI_TaxID; 28285; Adenovirus 5. CC Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) . CC Miscellaneous: Cell line information from personal communication of Yuste-Checa P. CC Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. OX NCBI_TaxID=9606; ! Homo sapiens (Human) HI CVCL_0063 ! HEK293T SX Female AG Fetus CA Transformed cell line Parental cell line: HEK293T (RRID:CVCL_0063)
HEK293E_Clu WT
By onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293T TetOff-SNCA-A53T mNeonGreen-3K-1-10-IRES-mKate2
By onID HEK293T TetOff-SNCA-A53T mNeonGreen-3K-1-10-IRES-mKate2 AC CVCL_D4BZ SY Syn10 split mNeonGreen CC Characteristics: Transduced with an all-in-one TetOff construct for the controllable expression of untagged SNCA p.Ala53Thr (from parent cell line). CC Characteristics: Additional expression of a plasmid encoding a bicistronic IRES construct of a split mNeonGreen (3K variant, B-strands 1-10) as well as an mKate2 marker (Direct_author_submission). CC Transfected with: HGNC; 11138; SNCA (with p.Ala53Thr). CC Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. CC Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). CC Transfected with: mKate2, a bright red fluorescent protein derived from eqFP578. CC Transfected with: UniProtKB; A0A1S4NYF2; mNeonGreen (derivative of Branchiostoma lanceolatum blFP-Y3). CC Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd). CC Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). CC Transfected with: UniProtKB; P00552; Transposon Tn5 neo. CC Transformant: NCBI_TaxID; 28285; Adenovirus 5. CC Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) . CC Miscellaneous: Cell line information from personal communication of Yuste-Checa P. CC Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. OX NCBI_TaxID=9606; ! Homo sapiens (Human) hi CVCL_D4BW ! HEK293T TetOff-SNCA-A53T SX Female AG Fetus CA Transformed cell line Parental cell line: HEK293T (RRID:CVCL_0063)
HEK293T TetOff-SNCA-A53T EGFP-RNF152-IRES-mScarlet-I
By onID HEK293T TetOff-SNCA-A53T EGFP-RNF152-IRES-mScarlet-I AC CVCL_D4BY SY Syn10 RNF152 reporter CC Characteristics: Transduced with an all-in-one TetOff construct for the controllable expression of untagged SNCA p.Ala53Thr (from parent cell line). CC Characteristics: Additional expression of a plasmid encoding a bicistronic IRES construct of EGFP-tagged RNF152, along with an mScarlet-I transfection marker (Direct_author_submission). CC Transfected with: HGNC; 26811; RNF152. CC Transfected with: HGNC; 11138; SNCA (with p.Ala53Thr). CC Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. CC Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). CC Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). CC Transfected with: mScarlet, a red fluorescent protein. CC Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd). CC Transfected with: UniProtKB; P00552; Transposon Tn5 neo. CC Transformant: NCBI_TaxID; 28285; Adenovirus 5. CC Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) . CC Miscellaneous: Cell line information from personal communication of Yuste-Checa P. CC Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. OX NCBI_TaxID=9606; ! Homo sapiens (Human) hi CVCL_D4BW ! HEK293T TetOff-SNCA-A53T SX Female AG Fetus CA Transformed cell line Parental cell line: HEK293T (RRID:CVCL_0063)
HEK293E_Clu TL1
By onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu TL3
By onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
