Therapeutic deep brain stimulation disrupts movement-related subthalamic nucleus activity in Parkinsonian mice
By Blythe Lloyd onPublication: Subthalamic nucleus deep brain stimulation relieves many motor symptoms of Parkinson’s disease, but its underlying therapeutic mechanisms remain unclear. Here, authors used electrical artifact-free GCaMP fiber photometry to investigate activity in basal ganglia nuclei during STN DBS in parkinsonian mice to get at that question. View original preprint.
GST Bead pulldown Assay
By Blythe Lloyd onGST Pulldown Assay for recruitment of bait proteins to GST labeled prey proteins. Prey proteins can be purified or from lysate.
Glucosylceramidase Beta (GBA) Genotyping
By Blythe Lloyd onThis protocol has been adapted from the PRoBaND Clinical Consortium (incorporating methods described by Neuman et al., 2009 and Stone et al., 2000) and has been used for all publications for PRoBaND / Tracking Parkinson's describing clinical data and outcomes with respect to GBA status.
Fiber Photometry (Mouse)
By Blythe Lloyd onThis protocol describes the procedure for fiber photometry in awake behaving mice. It includes details on the surgical implantation of fibers.
Expression and purification Twin-STREP-FLAG tagged ATG13:ATG101 constructs
By Blythe Lloyd onExpression and purification Twin-STREP-FLAG tagged ATG13:ATG101 constructs
Expression and purification protocol of WIPI2d
By Blythe Lloyd onThis protocol details the expression and purification protocol of WIPI2d.
Expression and purification protocol of GST-TAX1BP1
By Blythe Lloyd onThis protocol details the expression and purification protocol of GST-TAX1BP1.
Expression and purification protocol of GST-mCh-FYVE
By Blythe Lloyd onThis protocol details the expression and purification of GST-mCh-FYVE.
Expression and purification MBP-ATG9 Constructs
By Blythe Lloyd onExpression and purification from HEK cells of ATG13, ATG101 and FOLDON-ATG9A proteins.
Expression and purification GST-tagged ATG13:ATG101 constructs
By Blythe Lloyd onExpression and purification of GST-tagged ATG13/ATG101 constructs.
Ex Vivo Electrophysiology
By Blythe Lloyd onThis protocol describes preparation of brain slices, setup of electrophysiology rig, and solutions for collecting whole cell and cell attached recordings.
LRRK2 kinase activity regulates GCase level and enzymatic activity differently depending on cell type in Parkinson’s disease
By taliag onPublished: Recent work suggests that LRRK2 kinase activity can modulate glucocerebrosidase (GCase) function. The authors investigated the relationship between LRRK2 and GBA1 by assessing GCase activity and found a positive correlation between the activities of LRRK2 and GCase in different cellular and ex vivo models. View original preprint.
Electron microscope sample preparation technique_V2
By Blythe Lloyd onThere are eight main steps in preparing EM sample, including fixation, en bloc staining, agarose embedding, dehydration, infiltration, resin embedding, resin-embedded sample trimming and ultrathin sectioning. This EM sample preparation protocol is designed for processing cultured cell sample.
Design and preparation of synthetic reference peptides for APP/Aβ TOMAHAQ proteomics, version 2
By Blythe Lloyd onThis protocol describes the design and preparation of synthetic reference peptides for APP/Aβ TOMAHAQ proteomics.
Deep Brain Stimulation (DBS) Implant
By Blythe Lloyd onThis protocol describes the steps for making implants used for deep brain stimulation (DBS) in rodents.
Crystallization, data collection and structure solution of PxP-VAB
By Blythe Lloyd onProtocol lists the steps to obtain PxP(Mcp1ct)-VABct crystals, X-ray diffraction data acquisition and obtaining the structure solution using molecular replacement with a model generated by AlphaFold2.
Sample Collection and Measurement of Serum Neurofilament Light (NfL)
By Michelle onThis protocol details the steps for the preparation of serum from human blood samples and the measurement of NfL concentration using the NF-Light Advantage kit on the HD-X Analyzer (Quanterix, Billerica, MA).
Single-cell dissociation of brain organoids
By Michelle onSingle-cell dissociation of brain organoids