Ubiquitin immunoprecipitation using an anti-ubiquitin nanobody

Output Details

This protocol describes a method to detect ubiquitination on a protein of interest. This technique relies on immunoprecipitation (IP) of ubiquitinated proteins from a cell lysate through the use of an anti-ubiquitin nanobody coupled to agarose beads. The eluate can be run on SDS-PAGE to determine whether the protein of interest was recovered in the IP and, therefore, ubiquitinated.
Tags
  • Human
  • Immunoprecipitation
  • In Vitro
  • Nanobodies
  • Protein
  • Ubiquitin
  • Western Blot

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