Induction of aggregation in alpha-synuclein-expressing cells by treatment with preformed fibrils (PFFs)

Description

This protocol details how to efficiently produce alpha-synuclein aggregates in cells by templating the misfolding of intracellular alpha-synuclein through treatment with preformed fibrils of alpha-synuclein (PFFs).

Identifier (DOI)

10.17504/protocols.io.eq2lyjbbplx9/v1

Tags

Alpha-synuclein
Cell biology
Pre-formed fibrils (PFFs)
SNCA

Team

Team Harper

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Cole Sitron, PhD

Key Personnel: Team Harper,

Max Planck Institute of Biochemistry
Victoria Anne Trinkaus, MSc

Key Personnel: Team Harper,

Max Planck Institute of Biochemistry
Franz-Ulrich Hartl, MD

Co-PI (Core Leadership): Team Harper,

Max Planck Institute of Biochemistry

Induction of aggregation in alpha-synuclein-expressing cells by treatment with preformed fibrils (PFFs)

Output Details

Meet the Authors

Cole Sitron, PhD

Victoria Anne Trinkaus, MSc

Franz-Ulrich Hartl, MD

Related Research

Characterizing Parkinson’s Disease Clinical and Biomarker Interactions in REM Sleep Behavior Disorder

Raw tomogram of GFP-a-synuclein inclusion in primary mouse neuron expressing GFP-a-synuclein, seeded with PFFs

Raw tomogram of GFP-a-synuclein inclusion in primary mouse neuron expressing GFP-a-synuclein, seeded with MSA aggregates