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Induction of aggregation in alpha-synuclein-expressing cells by treatment with preformed fibrils (PFFs)

By Cole Sitron, PhD, Victoria Anne Trinkaus, MSc and Franz-Ulrich Hartl, MD
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Output Details

Description
This protocol details how to efficiently produce alpha-synuclein aggregates in cells by templating the misfolding of intracellular alpha-synuclein through treatment with preformed fibrils of alpha-synuclein (PFFs).
Identifier (DOI)
10.17504/protocols.io.eq2lyjbbplx9/v1
Tags
  • Alpha-synuclein
  • Cell biology
  • Pre-formed fibrils (PFFs)
  • SNCA
Team
Team Harper
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

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