Induction of aggregation in alpha-synuclein-expressing cells by treatment with preformed fibrils (PFFs)

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Description

This protocol details how to efficiently produce alpha-synuclein aggregates in cells by templating the misfolding of intracellular alpha-synuclein through treatment with preformed fibrils of alpha-synuclein (PFFs).

Identifier (DOI)

10.17504/protocols.io.eq2lyjbbplx9/v1

Tags

Alpha-synuclein
Cell biology
Pre-formed fibrils (PFFs)
SNCA

Discovery Team

Team Harper

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Cole Sitron

External Collaborator
Victoria Anne Trinkaus

External Collaborator
Franz-Ulrich Hartl, MD

Co-PI (Core Leadership): Team Harper,

Max Planck Institute of Biochemistry

Induction of aggregation in alpha-synuclein-expressing cells by treatment with preformed fibrils (PFFs)

Output Details

Meet the Authors

Cole Sitron

Victoria Anne Trinkaus

Franz-Ulrich Hartl, MD

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