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  • HEK293E_Clu TL2

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    ID   HEK293E_Clu TL2 AC   CVCL_D4BF CC   Characteristics: Transfected with the pB-T-PAF expression plasmid expressing mutated CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter (Direct_author_submission). CC   Transfected with: HGNC; 2095; CLU (with p.Tyr204Ser, p.Tyr206Ser, p.Leu207Ser, p.Phe209Ser, p.Leu211Ser, p.Phe218Ser, p.Phe219Ser and p.Phe220Ser). CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). CC   Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. CC   Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). CC   Transfected with: UniProtKB; P03211; Epstein-Barr virus (strain B95-8) EBNA1. CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5. CC   Miscellaneous: Cell line information from personal communication of Yuste-Checa P. CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. HI   CVCL_6974 ! HEK293-EBNA SX   Female AG   Fetus CA   Transformed cell line Parental cell line: HEK293T (RRID:CVCL_0063)

  • HEK293E_Clu TL4

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    Cell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.

  • HEK293E_Clu TL2+3

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    Cell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.

  • HEK293E_Clu CC2

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    Cell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.

  • HEK293E_Clu CC1

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    Cell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.

  • HEK293E_Clu CC4

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    ID   HEK293E_Clu CC4 AC   CVCL_D4BM CC   Characteristics: Transfected with the pB-T-PAF expression plasmid expressing mutated CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter (Direct_author_submission). CC   Transfected with: HGNC; 2095; CLU (with p.Ser161Arg). CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). CC   Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. CC   Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). CC   Transfected with: UniProtKB; P03211; Epstein-Barr virus (strain B95-8) EBNA1. CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5. CC   Miscellaneous: Cell line information from personal communication of Yuste-Checa P. CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. HI   CVCL_6974 ! HEK293-EBNA SX   Female AG   Fetus CA   Transformed cell line Parental cell line: HEK293T (RRID:CVCL_0063)

  • HEK293E_Clu CC3

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    Cell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.

  • HEK293E_Clu DD2

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    ID   HEK293E_Clu DD2 AC   CVCL_D4BP CC   Characteristics: Transfected with the pB-T-PAF expression plasmid expressing mutated CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter (Direct_author_submission). CC   Transfected with: HGNC; 2095; CLU (with p.Tyr125Phe, p.Gly132Glu, p.Leu135Lys, p.Arg138Glu, p.Gln139Ala and p.Glu142Ser). CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). CC   Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. CC   Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). CC   Transfected with: UniProtKB; P03211; Epstein-Barr virus (strain B95-8) EBNA1. CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5. CC   Miscellaneous: Cell line information from personal communication of Yuste-Checa P. CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. HI   CVCL_6974 ! HEK293-EBNA SX   Female AG   Fetus CA   Transformed cell line Parental cell line: HEK293T (RRID:CVCL_0063)

  • HEK293E_Clu DD1

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    Cell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.

  • HEK293E_Clu AB1

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    ID   HEK293E_Clu AB1 AC   CVCL_D4BR CC   Characteristics: Transfected with the pB-T-PAF expression plasmid expressing mutated CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter (Direct_author_submission). CC   Transfected with: HGNC; 2095; CLU (with p.Val26Asp, p.Met34Trp and p.Arg37Glu). CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). CC   Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. CC   Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). CC   Transfected with: UniProtKB; P03211; Epstein-Barr virus (strain B95-8) EBNA1. CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5. CC   Miscellaneous: Cell line information from personal communication of Yuste-Checa P. CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. HI   CVCL_6974 ! HEK293-EBNA SX   Female AG   Fetus CA   Transformed cell line Parental cell line: HEK293T (RRID:CVCL_0063)

  • HEK293E_Clu DD3

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    Cell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.

  • pAAV_hSyn_GCaMP6m_sWPRE_spA_mDLX_nls_mRuby3_ssWPRE_spA

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    It is a viral construct designed to express GCaMP6m pan-neuronally and specifically express a red fluorophore (mRuby) in the nucleus of cortical interneurons. This construct is ideal for dual-imaging experiments to capture neuronal activity of two neuronal populations with the sensitive blue-absorbing GCaMP6m (no light artifact) and to identify interneurons with red fluorescence in parallel or post-mortem.

  • HEK293E_Ecto short VLDLR-C-tag

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    Transfected with plasmid expressing the ectodomain of VLDLR fused to a C-terminal C-tag (EPEA) under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.

  • HEK293E_Clu AB2

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    Cell Line: Transfected with the pB-T-PAF expression plasmid expressing mutated CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.

  • pAAV_EF1a_DIO_jGCaMP8f_nano_ribo_WPRE

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    This links GCaMP8f with a ribosome-tethered nanobody that restricts GCaMP to the soma of neurons. Useful in very dense nuclei with strong neuropil, which can make post-hoc cell segmentation difficult.

  • pAAV_Ef1a_DIO_MyrPalm_mScarlet_WT_IRES_mNeon_minBack

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    Bright green (mNeon) and red (mRuby) fluorescence proteins are expressed, with the former being cytoplasmic and the latter membrane-targeted. This expression is ideal for tracking long diffuse axons in cortical structures in vivo or for slice histology and cleared brain tissues post-mortem. The construct is expressed under a strong EF1a promoter in a cre-dependent manner.

  • pAAV_Ef1a_DIO_hTyrNull_HATag

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    Strong expression of Ef1a promoter, HA tag for histological verification of expression.

  • pAAV_Cbh_DIO_hTyrNull_HATag

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    Strong expression of CbH promoter that is non-silencenable, HA tag for histological verification of expression.

  • pAAV_Cbh_DIO_hTyr

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    Cre-dependent expression of human tyrosinase induces the formation of neuromelanin in catecholaminergic neurons. The CbH promoter is a strong, pan-neuronal, non-silencable promoter.

  • pAAV_Ef1a_DIO_hTyrHA_minBack

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    Cre-dependent expression of human tyrosinase induces the formation of neuromelanin in catecholaminergic neurons. Expression under strong Ef1a promoter.

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