pAAV_EF1a_DIO_jGCaMP8f_nano_ribo_WPRE

Output Details

This links GCaMP8f with a ribosome-tethered nanobody that restricts GCaMP to the soma of neurons. Particularly useful in very dense nuclei with strong neuropil, which can make post-hoc cell segmentation (i.e. independent component analysis (ICA) or constrained nonnegative matrix factorization (CMNF)) difficult.
Tags
  • AAV
  • AAV (Adenoassociated Virus)
  • Live-imaging
  • Nanobodies
  • Two-Photon Microscopy