Immunofluorescence on human brain FFPE sections to identify neuromelanin in A6, A9, and A10
By onA protocol to scan brain neuromelanin with reduced impact of the chemicals on their original color and intensity. The immunofluorescence staining protocol is designed to identify human A6, A9, and A10 cell clusters and alpha-synuclein pathology.
pAAV_CbH_SIO_SyGCaMP8s_p2a_mRuby
By onThis construct links GCaMP8s to Synaptophysin and directs the fusion protein to presynaptic sites. It also produces a cytoplasmic red fluorescence protein (mRuby) via the self-splicing peptide 2A.
TruAI Neuromelanin Quantification
By onQuantification of area and optical density of intracellular neuromelanin with TruAI.
Preparing 10X Genomics CytAssist FFPE Sections
By onDesigning and properly mounting FFPE sections on large-window CytAssist slides is important for downstream analysis and data quality. This protocol describes the key steps for this preparation.
Mouse brain hemisphere organotypic cultures on glass coverslips
By onThis protocol describes preparation of organotypic hemisphere cultures prepared from mouse brain and cultured as rollerdrum cultures on glass coverslips.
Neuromelanin Processing and Image Acquisition
By onProtocol for preparing post-mortem tissue for intracellular NM quantification.
Immunophenotyping of immune cells by high dimensional flow cytometry
By onProtocol to assess cellular phenotypes by multiparametric flow cytometry panels containing markers to identify cell types and activation states.
Intracardiac perfusion and rat brain fixation for immunohistochemistry
By onProtocol for rat brain processing to perform parallel histological analysis in one hemisphere and high dimensional flow cytometry in the other.
Lipopolysaccharide intraperitoneal injection in rats and sickness behavior assessment
By onThis protocol is a quick guide to how to prepare a solution of LPS on the saline vehicle, how to inject a rat for an intraperitoneal injection, and how to monitor the animal after injection.
Immunofluorescence free-floating rat brain cryosections
By onProtocol for immunofluorescence on rat brain cryosections
Immunohistochemistry free-floating rat brain cryosections
By onProtocol for immunohistochemistry on rat brain cryosections.
Immunophenotyping of the peripheral blood immune cells by flow cytometry
By onProtocol for immunophenotyping peripheral blood immune cells.
Optical densitometry of tyrosine hydroxylase fibers
By onProtocol for quantifying TH+ fibers in rat striatum and substantia nigra pars reticulata (SNpr)
Stereology-mediated cell count using StereoInvestigator
By onProtocol for cell counting using StereoInvestigator software.
Constant Potential Amperometry in vitro
By onProtocol for Constant Potential Amperometry recordings in vitro of evoked dopamine release in the rat striatum
Open field test
By onThe open field test is a simple test to evaluate general motor and explorative behavior in rodents.
Systemic inflammation triggers long-lasting neuroinflammation and accelerates neurodegeneration in a rat model of Parkinson’s disease overexpressing human alpha-synuclein
By onIn the present study, the authors assessed the development of PD-like symptoms on a PD rat model overexpressing human α-synuclein at a presymptomatic age, exposed to a pro-inflammatory insult by injection of lipopolysaccharide.
Sucrose preference test
By onThe sucrose preference test assesses the animal’s interest in a sweet-tasting sucrose solution relative to unsweetened water.
