Mouse brain hemisphere organotypic cultures on glass coverslips
Output Details
Description
This protocol describes preparation of organotypic hemisphere cultures prepared from mouse brain and cultured as rollerdrum cultures on glass coverslips. Having the cultures directly attached to glass coverslips facilitates imaging at very high resolution on inverted microscopes, including time lapse imaging. We specifically use these for STED microscopy based super-resolution microscopy, including super-resolution shadow imaging (SUSHI).
Identifier (DOI)
10.17504/protocols.io.j8nlkojxwv5r/v1
