Nigrostriatal organotypic cultures to study neuromelanin accumulation in dopaminergic circuits
By onIn this protocol we describe the preparation and maintenance of rat organotypic cultures from parasagittal brain slices. We use a 13º slicing angle and a customized cocktail of small molecules and growth factors, to maximize the integrity of the nigrostriatal pathway and ensure the survival of dopaminergic neurons, respectively. The slices maintain the basic cytoarchitecture of the brain, including glia and the extracellular matrix (not including vessels nor immune system). The cultures can be used to study dopaminergic degeneration, cell-cell and cell-matrix interactions and are particularly suitable for AAV-mediated overexpression of transgenes, time-lapse live imaging and longitudinal studies.
Patient PBMC differentiation to macrophages
By onDifferentiation of patient peripheral blood mononuclear cells (PBMCs) into macrophages is a common method used in research to study immune responses and diseases.
Patient PBMC flow cytometry
By onFlow cytometry analysis of peripheral blood mononuclear cells (PBMC) from a patient was performed.
DQ-BSA and BMV109 microscopy
By onDQ-BSA and BMV109 microscopy used for studying peritoneal macrophages.
pH-Rodo pathogens to monitor phagocytosis in pMacs
By onpH-Rodo pathogens are used to track phagocytosis in peritoneal macrophages (pMacs).
Harvesting and culturing of peritoneal macrophages and ex-vivo stimulation
By onThe process involves harvesting and culturing peritoneal macrophages followed by ex-vivo stimulation with IFNy for research purposes.
Flow cytometry for ex vivo stimulated pMacs
By onFlow cytometry used to analyze ex vivo stimulated peritoneal macrophages for research purposes.
Ea(52-68) uptake assays
By onEa(52-68) uptake assays were performed on peritoneal macrophages (pMacs).
Primary data associated with the manuscript 10.1126/science.adi9926 (“Rab29-dependent asymmetrical activation of leucine-rich repeat kinase 2”)
By onPrimary data associated with the manuscript "Rab29-dependent asymmetrical activation of leucine-rich repeat kinase 2".
Loss of primary cilia and dopaminergic neuroprotection in pathogenic LRRK2-driven and idiopathic Parkinson’s disease
By onActivating LRRK2 mutations causes Parkinson's disease. Here, the data strongly suggest that loss of cilia in specific striatal cell types decreases neuroprotection for dopamine neurons in mice and human Parkinson's disease.
Evaluating GPNMB ACD mutants by Western Blotting and immunofluorescence
By onThis protocol describes the evaluation of cellular processing of GPNMB mutants by Western Blotting and Immunofluorescent imaging in a HEK293 overexpression model.
Neural activation of the GI tract
By onProtocol for neuronal activation of the enteric nervous system.
GCaMP6f Fluorescence in Ex Vivo Intestinal Preparation
By onProtocol for GCaMP6f imaging in ex vivo mouse intestinal tissue.
Tissue preparation, immunohistochemistry, Imaging, and Quantification
By onProtocol for neuronal imaging
Metabolomics Preparation and Analysis
By onProtocol for metabolomic analysis from mouse fecal and cecal samples.
Metagenome Preparation and Analysis
By onProtocol for metagenomic analysis from mouse fecal pellets.
Whole Gut Transit Time, Fecal Water Content, and Fecal Output
By onProtocol for mouse gastrointestinal function assays (whole gut transit time, fecal water content, and fecal output)
