HEK293T TetOff-SNCA-A53T mNeonGreen-3K-1-10-IRES-mKate2
Output Details
Description
ID HEK293T TetOff-SNCA-A53T mNeonGreen-3K-1-10-IRES-mKate2
AC CVCL_D4BZ
SY Syn10 split mNeonGreen
CC Characteristics: Transduced with an all-in-one TetOff construct for the controllable expression of untagged SNCA p.Ala53Thr (from parent cell line).
CC Characteristics: Additional expression of a plasmid encoding a bicistronic IRES construct of a split mNeonGreen (3K variant, B-strands 1-10) as well as an mKate2 marker (Direct_author_submission).
CC Transfected with: HGNC; 11138; SNCA (with p.Ala53Thr).
CC Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
CC Transfected with: mKate2, a bright red fluorescent protein derived from eqFP578.
CC Transfected with: UniProtKB; A0A1S4NYF2; mNeonGreen (derivative of Branchiostoma lanceolatum blFP-Y3).
CC Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
CC Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [Note=tsA].
CC Miscellaneous: Cell line information from personal communication of Yuste-Checa P.
CC Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX NCBI_TaxID=9606; ! Homo sapiens (Human)
hi CVCL_D4BW ! HEK293T TetOff-SNCA-A53T
SX Female
AG Fetus
CA Transformed cell line
Parental cell line: HEK293T (RRID:CVCL_0063)
