Presynaptic autophagy is coupled to the synaptic vesicle cycle via ATG-9
By Blythe Lloyd onPublished: ATG9 is a core autophagy transmembrane protein present at nerve terminals. The authors found that ATG9 is a component of vesicles that undergo exo-endocytosis at synapses and that synaptojanin 1 mutations disrupt ATG9 activity at synapses. View original preprint.
VPS13D bridges the ER to mitochondria and peroxisomes via Miro
By Blythe Lloyd onPublished: VPS13C mutations are implicated in PD. The authors found that VPS13D, a closely related protein, can mediate a bridge between the peroxisome and different organelles (ER or mitochondria). Authors identified the specific ER and mitochondrial proteins facilitating this interaction resulting in dysregulation of lipid flux between them. View original preprint.
Reconstitution of cargo-induced LC3 lipidation in mammalian selective autophagy
By taliag onGUV quantification
ALS and FTD-associated missense mutations in TBK1 differentially disrupt mitophagy
By taliag onRaw data associated with DOI: 10.1073/pnas.2025053118
Parkinson’s disease and cancer: a systematic re and meta-analysis of over 17 million participants
By Blythe Lloyd onPublished: The authors examined risk association between Parkinson’s disease and cancer using data from 63 publications, totaling in 17 million individuals. With the exception of melanoma, the authors found that the risk association of Parkinson’s disease and cancer was inversely related.
Microscopy-based bead protein-protein interaction assay
By taliag onAn assay to study protein-protein interaction
Assessing enrichment of proteins in the mitochondrial fraction in HEK cells
By taliag onThis is a method for measuring protein enrichment on mitochondria in various conditions. In the resulting Western blot, one can assess the level of contamination of other organelles in the enrichment prep.
Structural basis for membrane recruitment of ATG16L1 by WIPI2 in autophagy
By taliag onPublished: Autophagy is a conserved mechanism for the sequestration and degradation of cytosolic cargo. ATG16L1 and WIPI2 are essential for autophagy initiation. The authors showed through structural determination how ATG16L1 and WIPI2 interact and compared the other WIPI proteins showing the variety of mechanisms of membrane recruitment by WIPI proteins. View original preprint.
Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1
By Blythe Lloyd onPublished: Loss-of-function mutations in Parkin cause disruption of mitophagy and are associated with PD. Yet, much of the biology surrounding Parkin function has taken place in artificial cell systems. The authors used human neurons to identify and validate 22 protein targets of Parkin, providing a functional Parkin landscape in neuronal cells.