Standard operating procedure (SOP) for systemic administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in non-human primates
By savannah onThis protocol details standard operating procedure (SOP) for systemic administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in non-human primates.
Preparation of LRRK1 RCKW cryo-EM grids
By savannah onProtocol used to create LRRK1 RCKW grids for cryo-EM used in Snead, Matyszewski, Dickey et al.
Regulation of mitophagy by the NSL complex underlies genetic risk for Parkinson’s disease at 16q11.2 and MAPT H1 loci
By savannah onThese results enrich our understanding of physiological events regulating mitophagy and establish a novel pathway for drug targeting in neurodegeneration.
Impaired dopamine release in Parkinson’s disease
By savannah onEvidence indicates that impaired dopamine release can result from disruption to a diverse range of Parkinson’s disease-associated genetic and molecular disturbances, and can be considered as a potential pathophysiological hallmark of Parkinson’s disease.
Unconventional secretion of α-synuclein mediated by palmitoylated DNAJC5 oligomers
By savannah onThe secretion of endogenous α-syn mediated by DNAJC5 is also found in a human neuroblastoma cell line, SH-SY5Y, differentiated into neurons in the presence of retinoic acid, and in human-induced pluripotent stem cell-derived midbrain dopamine neurons. We propose that DNAJC5 forms a palmitoylated oligomer to accommodate and export α-syn.
From Policy to Practice: Tracking an Open Science Funding Initiative
By savannah onBy normalizing the open science and compliance process across funding bodies, we hope to simplify and streamline researcher, institutional, and funder workflows, allowing researchers to focus on science by easily leveraging resources and building upon the work of others.
Analysis of glycosphingolipids from human cerebrospinal fluid
By savannah onThe method uses the fluorescent compound anthranilic acid (2-AA) to label oligosaccharides prior to analysis using normal-phase high-performance liquid chromatography. The labeling procedure is rapid, selective, and easy to perform. With the inclusion of a 2AA-labeled chitotriose calibration standard, it is possible to obtain accurate and reproducible molar quantities of individual GSL species.
Genome-wide screen reveals Rab12 GTPase as a critical activator of Parkinson’s disease-linked LRRK2 kinase
By savannah onPublished: The data supports a model in which Rab12 binding to a new site in the LRRK2 Armadillo domain activates LRRK2 kinase for Rab phosphorylation and could serve as a new therapeutic target for a novel class of LRRK2 inhibitors that do not target the kinase domain. View original preprint.
cDNA clones for expression of MitoTag (OMP25) in mammalian cells
By savannah onPlasmids for expression of OMP25 (MitoTag) in human and mouse cells.
cDNA clones for expression of LysoTag (TMEM192) in mammalian cells
By savannah onPlasmids for expression of TMEM192 (LysoTag) in human and mouse cells.
cDNA clones for expression of GolgiTag (TMEM115) in mammalian cells
By savannah onPlasmids for expression of TMEM115 (GolgiTag) in human and mouse cells.
mCh-Miro1(∆TM)-SspB
By savannah onmCh-Miro1(∆TM)-SspB plasmid has a deletion of transmembrane domain and is used for improved light inducible dimer assay.
Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function
By savannah onGlucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function. Collection of protocols for paper: "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function."
Cryosectioning mouse brain
By savannah onThis protocol details the cryosectioning of the mouse brain.
Plasmid-reprogramming of human iPSCs
By savannah onThis protocol details about plasmid-reprogramming of human fibroblasts.
Protein network analysis links the NSL complex to Parkinson’s disease via mitochondrial and nuclear biology
By savannah onA bioinformatics approach was taken to investigate the proteome of the NSL complex, to unpick its relevance to PD progression. The authors’ data points to NSL complex members OGT and WDR5 as key drivers of this increased PD association. These findings strengthen a role for mitochondrial quality control in both familial and sporadic disease.
AAVS1-SA-neo-CAGGS-PE2-2A-GFP
By savannah onThis resource can be used to generate knock-in cell line faciliating prime editing.
A prebiotic diet modulates microglia response and motor deficits in α-synuclein overexpressing mice (Dataset)
By savannah onThese studies uncover a novel microglia-dependent interaction between diet and motor symptoms in mice, findings that may have implications for neuroinflammation and PD.
ASAP Blueprint for Collaborative Open Science
By savannah onThis Blueprint presents initial findings on how ASAP’s approach to open science has solidified and evolved over its first three years, data and metrics on progress, and CC-BY versions of assets that can be adopted and adapted by others. ASAP plans to update the Blueprint with new findings and assets on a regular basis.
Brain repair by cell replacement via in situ neuronal reprogramming
By savannah onThis review focuses on key advances in generating new neurons through in situ neuronal reprogramming, which is tied to fundamental questions regarding adult neurogenesis, cell source, and mechanisms for neuronal reprogramming, as well as the ability of new neurons to integrate into the existing circuitry.
