Immunofluorescence and object-based colocalization analysis
By onProcedures for immunofluorescence and object-based colocalization analysis used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
Hippocampal Cultures, lentivirus production, vGLUT1-pHluorin imaging and analysis
By onProcedures for hippocampal cultures, lentivirus production, vGLUT1-pHluorin imaging and analysis used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
Proximity Ligation Assay (PLA)
By onProcedure for proximity ligation assay used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
Electron microscopy (EM) and quantification of inter-vesicular distances
By onProcedure for electron microscopy (EM) and quantification of inter-vesicular distances used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
αSyn Immunohistochemistry
By onProcedure for immunohistochemistry used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
CRISPR nuclease (CRISPRn) genome editing
By onProcedure for CRISPR nuclease (CRISPRn) genome editing used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
CRISPR knock-in validation
By onProcedure for CRISPR knock-in validation used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
CRISPR knock-in (endogenous tagging)
By onProcedure for CRISPR knock-in (endogenous tagging) used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
Preparation of brain and Neuro2A lysates for biochemical assays and evaluation
By onProcedure for preparing brain and Neuro2A lysates for biochemical assays and evaluation used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
In vitro dephosphorylation
By onProcedure for in vitro dephosphorylation used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
Preparation of an Enriched Synaptic Vesicle Fraction
By onProcedure for preparing enriched synaptic vesicle fractions used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
In vitro phosphorylation
By onProcedure for in vitro phosphorylation used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function"
Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS)
By onProcedure for Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS) used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function"
Proteomics: On-bead/in-solution digestion and phosphopeptide enrichment
By onProcedure for On-bead/in-solution digestion and phosphopeptide enrichment used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function"
Multi-electrode array (MEA)
By onProcedure for multi-electrode array used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function"
Proteome analysis
By onProcedure for proteome analysis used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function"
ColabFold Protein Structure Prediction and Membrane Interaction Modeling
By onProcedure for ColabFold protein structure prediction and membrane interaction modeling used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function"
Multiple Sequence Alignment
By onProcedure for multiple sequence alignment used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function"
Day2023 dataset
By onDatasets for all figures 1-8 in: GABAergic regulation of striatal spiny projection neuron excitability depends upon their activity state. Image is taken from Fig 1B inset and illustrates RiboTag-eGFP in SPNs of the striatum.
TX2P – Transcript to protein
By onTX2P automates mass spectrometry data integration in long-read RNA-sequencing by predicting open reading frames, translating them into peptides, and searching for proteins in mass spec datasets with MetaMorpheus.
