Gene editing of YIPF4, FIP200, and ATG7 in HEK293 cells - Aligning Science Across Parkinson's

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Description

This protocol describes the creation of YIPF4, FIP200, and ATG7 knockout cell lines in HEK293 cells using CRISPR-Cas9.

Identifier (DOI)

10.17504/protocols.io.6qpvr3462vmk/v1

Tags

Autophagy
CRISPR

Discovery Team

Team Harper

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

J. Wade Harper

Lead PI (Core Leadership): Team Harper,

Harvard University
Sharan Swarup

External Collaborator
Kelsey Hickey

External Collaborator