fcs files to “The extracellular chaperone Clusterin enhances Tau aggregate seeding in a cellular model”
By Blythe Lloyd onDataset associated with DOI: 10.17617/3.51VXU0
The remote assessment of parkinsonism supporting the ongoing development of interventions in Gaucher disease
By taliag onPublished: This methodology report assesses a large cohort of GBA mutation carriers with the goal of developing procedures to diagnose PD earlier. View original preprint.
APEX-ATG8 proteomics with and without lysosomal damage
By Blythe Lloyd onProteomics data associated with DOI: 10.7554/eLife.72328
APEX-Galectin proteomics with and without lysosomal damage
By Blythe Lloyd onProteomics data associated with DOI: 10.7554/eLife.72328
Protocol for hippocampal neuronal cultures
By Blythe Lloyd onThis protocol details the procedure for preparation of neuronal cultures from mice hippocampi as it was performed in https://doi.org/10.1083/jcb.202010004 but can also be used to prepare cultures of cortical neurons.
APEX-autophagy cargo receptor proteomics with and without lysosomal damage
By Blythe Lloyd onProteomics data associated with DOI: 10.7554/eLife.72328
Microbes and Parkinson’s Disease: from associations to mechanisms.
By Michelle onReview: This review dissects the microbial contributions to PD with a particular focus on recent advances and rodent models that can be used for microorganism hypothesis testing.
Structural Biology of LRRK2 and its Interaction with Microtubules
By taliag onReview: This review focuses on new insights into the stucture of LRRK2's cytosolic and microtubule-bound forms and challenges going forward.
Optogenetic experiments with iLID system
By Michelle onThis protocol details experiments with the iLID optogenetic system as performed to acutely recruit Miro to mitochondria in https://doi.org/10.1083/jcb.202010004.
Lyso-IP proteomics with and without lysosomal damage
By Blythe Lloyd onProteomics data associated with DOI: 10.7554/eLife.72328
Protocol for Image processing and analysis of VPS13D recruitment to mitochondria
By Michelle onThis protocol details the image processing and analysis of VPS13D recruitment to mitochondria as it was performed in https://doi.org/10.1083/jcb.202010004. The first part details the analysis of acute optogenetic recruitment, and the second part of basal recruitment under different conditions.
The extracellular chaperone Clusterin enhances Tau aggregate seeding in a cellular model
By Michelle onPublished: Tau neuronal aggregation is a driver of some neurodegenerative disorders. These toxic aggregates form when affected cells release aggregate seeds which are then internalized by unaffected cells. The authors show that a protein,Clusterin, delays Tau aggregation and suppresses seeding activity. View original preprint.
The Hsc70 disaggregation machinery removes monomer units directly from α-synuclein fibril ends
By Michelle onPublished: Irreversible toxic aggregates are a hallmark of neurodegenerative diseases, such as alpha-synuclein in PD. Using microfluidic diffusional sizing, the authors show that the molecular chaperone family Hsp70 (specifically Hsc70, DnaJB, and Apg2) can completely dissolve alpha-synuclein aggregation and revert it back to its monomeric state.
Quantitative proteomics reveals the selectivity of ubiquitin-binding autophagy receptors in the turnover of damaged lysosomes by lysophagy
By Michelle onPublished: The authors used proteomics to develop a quantitative snapshot of the proteins involved in lysophagy. Among the proteins identified, they found that TAX1BP1 and TBK1 are both required for lysophagy. View original preprint.
Hippocampal Neuronal Culture
By Blythe Lloyd onThis protocol describes the procedure for hippocampal neuronal cultures from new - born mouse pups.