pU6-pegRNA-LRRK2-G2019S-3d
By quincy.tichenor onThis resource can be used to introduce LRRK2-G2019S mutation using prime editing.
Live-cell imaging; mitochondrial reactive oxygen species
By quincy.tichenor onThis protocol presents instructions for assessing mitochondrial souced reactive oxygen species using MitoTracker® Red CM-H2XRos dye (Thermo Fisher Scientific), a reduced non-fluorescent version of Mitotracker red that is fluorescent upon oxidation within mitochondria, which accumulates in mitochondria upon oxidation.
HeLa S3 penta KO-TBK1/ULK1/ULK2 TKO
By quincy.tichenor onHeLa S3 penta KO-TBK1/ULK1/ULK2 TKO cell line.
pGBdest-10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1_F32A-I35A-I36A_-GFP-TEV-StrepII
By quincy.tichenor onPlasmid for the Expression of the E3 complex with an FII (F32A, I35A, and I36A) mutation. SMC Internal reference: SMC1728
BPK1520-LRRK2-G2019S-ng
By quincy.tichenor onThis resource can be used to introduce LRRK2-G2019S mutation using prime editing, in PE3 approach.
Mechanisms controlling selective elimination of damaged lysosomes
By quincy.tichenor onReview: This review discusses the current state of our understanding of mechanisms used to mark and eliminate damaged lysosomes, and discuss the complexities of galectin function and ubiquitin-chain linkage types. Authors also discuss the limitations of available data and challenges with the goal of understanding the mechanistic basis of key steps in lysophagic flux.
P5B-ATPases in the mammalian polyamine transport system and their role in disease
By quincy.tichenor onReview: This review brings together the current knowledge of the cellular function of the mammalian polyamine transport system, focusing on the role of P5B-ATPases ATP13A2-5.
Astrocyte extraction from brain organoids V.2
By taliag onProtocol for astrocyte extraction from brain organoids.
Lysosome proteolysis analysis with DQ-BSA
By taliag onProtocol to analyze lysosomal proteolysis in astrocytes generated from iPSCs.
pLenti HsATP10B_D433N-T2A-His-flag-TMEM30A
By taliag onTransfer plasmid for lentiviral vector production expressing Hs ATP10B D433N mutant and His/flag tagged Hs TMEM30A.
pLenti HsATP10B_WT-T2A-His-flag-TMEM30A
By taliag onTransfer plasmid for lentiviral vector production expressing Hs ATP10B WT and His/Flag tagged Hs TMEM30A.
Probedesign pipeline for the inhouse generation of seqFISH probes
By taliag onThe probe design pipeline works by inputting the transcript id's for which probes have to be designed. Additional probe design parameters such as GC% or melting temperature can be changed via the config file. The pipeline will output the designed probes in fasta format and a csv file with the results of all filtering steps.
Basal ganglia neurons in healthy and parkinsonian primates generate recurring sequences of spikes (Dataset)
By taliag onThe data are interspike interval measurements generated from electrophysiological recordings of spontaneous spiking activities of single neurons in the basal ganglia of awake monkeys, before and after rendering them parkinsonian by treatment with MPTP. The animals were not engaged in any task or other structured activity.
Light microscopy immunoperoxidase staining
By taliag onProtocol for Light microscopy immunoperoxidase staining.
Bulk NGS/allele quantification – Highly efficient generation of isogenic pluripotent stem cell models using prime editing
By taliag onThis file contains sequencing results for amplicons covering specific regions of interest related to prime editing of hPSCs.
Midbrain organoid generation from mfNPC
By quincy.tichenor onMidbrain organoid generation from mfNPC.
