Immunochemistry on paraffin sections
By quincy.tichenor onProtocol for immunochemistry on paraffin sections.
Intracellular neuromelanin quantification
By quincy.tichenor onProtocol for intracellular neuromelanin quantification.
Rat brain processing for histological analyses
By quincy.tichenor onProtocol for rat brain processing for histological analyses.
Immunofluorescence on paraffin sections
By quincy.tichenor onProtocol for immunofluorescence on paraffin sections.
Determination of free and protein-bound DA and NE and their metabolites and oxidation products by UPLC-MS/MS method
By quincy.tichenor onProtocol for the determination of free and protein-bound DA and NE and their metabolites and oxidation products by UPLC-MS/MS method.
Stereology-mediated cell counting using StereoInvestigator
By quincy.tichenor onProtocol for stereologic count with StereoInvestigator.
Gene expression analysis by qPCR
By quincy.tichenor onProtocol for gene expression analysis by quantitative Real-Time PCR (qPCR).
RNA and protein extraction from bulk dissections
By quincy.tichenor onProtocol for RNA and protein extraction from bulk dissections.
The GBA variant E326K is associated with alpha-synuclein aggregation and lipid droplet accumulation in human cell lines
By quincy.tichenor onThe GBA variant E326K is associated with alpha-synuclein aggregation and lipid droplet accumulation in human cell lines. Associated with publication 10.1101/2022.06.01.494130.
Validation of genotyping method for L444P mice ear-clips
By quincy.tichenor onThe genotyping is used to identify if mice are heterozygote (hetero) or Wild-Type (WT). The aim of the work is to validate the digestion method, and PCR program, the PCR primers, and the interpretation of the results. Associated with publication: doi: 10.1093/brain/awx221.
fMRI tasks to explore emotion-motor interactions
By quincy.tichenor onCode for fMRI tasks designed to explore emotion-motor interactions—Active Escape and Approach-Avoidance tasks.
Coating coverslips for cell culture
By quincy.tichenor onCoated coverslips provide a nourishing adherent surface for cell culture. This protocol provides step by step instruction on how to coat coverslips for cell culture.
Data set of the manuscript “Neuronal hyperactivity-induced oxidant stress promotes in vivo a-synuclein brain spreading”
By quincy.tichenor onData set of the manuscript "Neuronal hyperactivity-induced oxidant stress promotes in vivo a-synuclein brain spreading".
Perfusion-fixation procedure for adult rhesus monkeys
By quincy.tichenor onPerfusion-fixation procedure for adult rhesus monkeys.
Midbrain organoid differentiation in spinner flasks
By quincy.tichenor onMidbrain differentiation protocol using spinner flasks.
Px330-EGFP-LRRK2-CRISPR/Cas9
By quincy.tichenor onThis resource can be used to introduce LRRK2-G2019S mutation using CRISPR/Cas9 based genome editing.
pU6-pegRNA-LRRK2-G2019S-3a
By quincy.tichenor onThis resource can be used to introduce LRRK2-G2019S mutation using prime editing.
pU6-pegRNA-LRRK2-G2019S-3b
By quincy.tichenor onThis resource can be used to introduce LRRK2-G2019S mutation using prime editing.
pU6-pegRNA-LRRK2-G2019S-3c
By quincy.tichenor onThis resource can be used to introduce LRRK2-G2019S mutation using prime editing.
