PTEN-induced kinase 1 (PINK1) and Parkin: Unlocking a mitochondrial quality control pathway linked to Parkinson’s disease
By Blythe Lloyd onReview: This review focuses on understanding the PINK1/Parkin-mediated mitochondrial quality control pathway through the lens of abherrant immune activation as a driver of dopaminerigic neuron loss following the loss of PINK and Parkin.
Rodent models based on endolysosomal genes involved in Parkinson’s disease
By Blythe Lloyd onReview: This review summarizes parkinsonian phenotypes in rodent models targeting genes that have a role in endolysosomal pathways and future steps to better understand the contribution of endolysosomal dysfunction to PD.
Lysosomal dysfunction in neurodegeneration: emerging concepts and methods
By taliag onReview: This review summarizes key technological advances that have led to a better understanding of the contribution of the lysosome to neurodegeneration and highlights key questions to be addressed moving forward.
The lysosome as a master regulator of iron metabolism
By taliag onReview: This review focuses on the role that the lysosome plays in maintaining iron homeostasis and how lysosomal iron dysregulation contributes to disease.
Western blot protocol for detecting ATP10B in mouse/rat brain
By Michelle onThis protocol describes the procedure for detection of ATP10B in rat and mouse brain tissue by Western blotting
ATP13A2-mediated endo-lysosomal polyamine export counters mitochondrial oxidative stress
By taliag onPublished: Loss-of-function of ATP13A2, an endo-lysosomal transporter that pumps polyamines into the cytosol, is associated with PD. ATP13A2 dysfunction causes polyamine accumulation within the lysosome and lysosomal rupture. The authors found a conserved cellular protective pathway involving ATP13A2-mediated lysosomal spermine export to provide protection against mitochondrial toxins.
ATP13A2 Regulates Cellular α-Synuclein Multimerization, Membrane Association, and Externalization
By taliag onPublished: ATP13A2 loss-of-function mutations cause lysosomal deficiency and are linked to Parkinson’s disease and alpha-synuclein pathology. The authors found that loss of ATP13A2 disrupts lysosomal membrane integrity and causes alpha-synuclein multimerization. Further, they showed that increased levels of ATP13A2 had a protective effect on alpha-synuclein aggregation.
Subcellular proteomics of dopamine neurons in the mouse brain
By taliag onPublished: Understanding the proteome of dopamine neuron is difficult due to the complex cytoarchitecture of the neurons. The authors were able to map the somatodendritic and axonal proteomes of midbrain dopaminergic neurons. Interestingly, the authors found striatal dopaminergic neurons house most proteins. View original preprint.