pBPK1520-SNCA-A53T-ng
By quincy.tichenor onIt can be used to introduce SNCA-A53T mutation using prime editing, PE3 approach.
pBPK1520-SNCA-A30P-ng
By quincy.tichenor onIt can be used to introduce SNCA-A30P mutation using prime editing, PE3 approach.
Highly efficient generation of isogenic pluripotent stem cell models using prime editing – Datasets
By quincy.tichenor onThis collection contains the following datasets related to the paper "Highly efficient generation of isogenic pluripotent stem cell models using prime editing." 1. AAVS1 knock-in genotyping 2. aCGH karyotyping 3. Tabular datasets for associated graphical figures
pGST2-GST-TEV-OPTN S177D S473D
By quincy.tichenor onPlasmid for the expression and purification of GST-TEV-OPTN S177D S473D. Internal Ref: SMC1588
pFastBac_Dual_GST-TEV-EGFP-TBK1
By quincy.tichenor onPlasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631
NDP52 and OPTN S177D S473D: expression and purification
By quincy.tichenor onThis protocol describes how to express and purify human NDP52 and OPTN S177D S473D. The same procedure can be applied to purify wild type OPTN.
pCAG-OSF-ATG13 (2-197)-Y118D
By quincy.tichenor onExpresses human ATG13 HORMA Y118D mutant in mammalian cells.
pCAG-OSF-ATG13 (2-197)-Y152D
By quincy.tichenor onExpresses human ATG13 HORMA Y152D mutant in mammalian cells.
pCAG-OSF-ATG13 (2-197)-Y115D
By quincy.tichenor onExpresses human ATG13 HORMA Y115D mutant in mammalian cells.
pCAG-OSF-ATG13 (2-197)-W50D
By quincy.tichenor onExpresses human ATG13 HORMA W50D mutant in mammalian cells.
pCAG-OSF-ATG13 (2-197)-R153D
By quincy.tichenor onExpresses human ATG13 HORMA R153D mutant in mammalian cells.
pCAG-OSF-ATG13 (2-197)-L151D
By quincy.tichenor onExpresses human ATG13 HORMA L151D mutant in mammalian cells.
pCAG-OSF-ATG13 (2-197)-K18D
By quincy.tichenor onExpresses human ATG13 HORMA K18D mutant in mammalian cells.
pCAG-OSF-ATG13 (2-197)-K15D
By quincy.tichenor onExpresses human ATG13 HORMA K15D mutant in mammalian cells.
pCAG-OSF-ATG13 (2-197)-F19D
By quincy.tichenor onExpresses human ATG13 HORMA F19D mutant in mammalian cells.
pCAG-OSF-ATG13 (2-197)-F16D
By quincy.tichenor onExpresses human ATG13 HORMA F16D mutant in mammalian cells.
pCAG-OSF-ATG13 (2-197)-E83L
By quincy.tichenor onExpresses human ATG13 HORMA E83L mutant in mammalian cells.
pCAG-OSF-ATG13 (2-197)
By quincy.tichenor onTo make ATG101/ATG13 HORMA complex for expression in mammalian cells.
pCAG-MBP-Foldon-ATG9 (830-839)
By quincy.tichenor onTo make ATG9 C-terminal tail trimer for expression in mammalian cells.
pCAG-MBP-Foldon-ATG9 (828-839)
By quincy.tichenor onTo make ATG9 C-terminal tail trimer for expression in mammalian cells.