RAB3 phosphorylation by pathogenic LRRK2 impairs trafficking of synaptic vesicle precursors
By onThe authors’ results reveal a mechanism by which pathogenic hyperactive LRRK2 may contribute to the altered synaptic homeostasis associated with characteristic non-motor and cognitive manifestations of PD.
Immunofluorescent labeling of cultured cells
By onThis protocol describes the process of Paraformaldehyde fixation and immunofluorescent-labeling of cultured cells.
Intracellular Cytokine (ICS) Staining Protocol
By onThis protocol details about intracellular cytokine (ICS) staining.
Pluripotency markers staining
By onThis protocol describes the standard procedure for staining pluripotency markers, e.g., OCT4, SSEA4, alkaline phosphatase, and etc. on human pluripotent stem cells (hPSCs).
Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures–Fluorospot SFC
By onFluorospot SFC (combined IFNg, IL-5, and IL-10) data and corresponding HC-PD group IDs described in "Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures"
Immunofluorescence assay (IFA)
By onThis protocol details the procedure of immunofluorescence assay (IFA).
Cryo-ET of S. cerevisiae during starvation (tomograms)
By onCryo-ET of S. cerevisiae during starvation (tomograms).
Membrane interaction and mechanism of LC3 lipidation machinery in autophagy – Raw GUV data
By onRaw GUV data of fluorescent protein imaged on a Nikon A1 confocal microscope with a 63 × Plan Apochromat 1.4 NA objective. Three biological replicates were performed for each experimental condition. Identical laser power and gain settings used.
Optogenetic experiments with iLID system
By onThis protocol details experiments with the iLID optogenetic system as performed to acutely recruit Miro to mitochondria.
3D-correlative FIB-milling and Cryo-ET of Autophagic structures in Yeast Cells
By onThis protocol describes how to plunge-freeze yeast on EM grids and how to target autophagic structures by combining cryo confocal fluorescence data to FIB-milling and tomogram acquisition.
Expansion Microscopy
By onExpansion microscopy is a technique to visualize biological structures with higher spatial resolution than traditional microscopy methods.
Unconventional Initiation of PINK1/Parkin Mitophagy by Optineurin
By onCargo sequestration is a fundamental step of selective autophagy in which cells generate a double membrane structure termed an autophagosome on the surface of cargoes. The authors uncover an unconventional path of PINK1/Parkin mitophagy initiation.
Live-cell imaging for synaptic vesicle precursors in human iNeuron axons
By onThe authors describe procedure and equipment used for live-imaging of synaptic vesicle precursors.
Live-cell imaging
By onLive-cell imaging is a technique to visualize dynamic cellular processes in living biological samples.
Image processing of full-length monomeric LRRK2
By onThis protocol assumes that 2000 to 4000 movies of full-length LRRK2 embedded on vitreous ice are collected with an electron microscope equipped with a direct detector. It focuses primarily on the monomeric population of LRRK2 and has been leading to 3-4 Å resolution structures.
Quick guide to use paceTOMO for cryo-ET data collection from Titan Krios
By onThis quick guide provides key minimal steps for preparing the Titan/SerialEM for the tomogram data collection on lamella or in vitro specimens with a K3 camera. paceTOMO routine is also included for a typical tomogram data collection session. Please note that this is not an exhaustive guide, but summarises the order of key steps.
Structural basis for ATG9A recruitment to the ULK1 complex in mitophagy initiation
By onHere, the authors examine the structural interaction between ATG9A and components of the ULK1 complex to better understand the process of the PINK1- and Parkin- dependent mitophagy pathway implicated in Parkinson's disease.
Microscopy-based evaluation of Parkin translocation and mitophagy in FBXO7-/- cell lines
By onAssociated with preprint:
