SoRA microscopy protocol for imaging oligomers in human brain tissue
By onThis protocol gives a step-by-step guide to imaging oligomers in human brain tissue using a spinning disk confocal microscope.
Raw tomogram of GFP-a-synuclein inclusion in primary mouse neuron expressing GFP-a-synuclein, seeded with PFFs
By onRaw data associated with DOI: 10.1038/s41467-021-22108-0
Fixation and imaging of HeLa cells after mitochondrial depolarization
By onA protocol for the fixation and imaging of HeLa cells after mitochondrial depolarization
Subtomogram averaging and classification
By onThis protocol describes the procedure of subtomogram averaging and 3D classification of VPS13C rod-shaped densities inside cryotomograms of mammalian cells. Sub-tomogram averaging package i3 was used for 3D alignment and classification.
Raw tomogram of GFP-a-synuclein inclusion in primary mouse neuron expressing GFP-a-synuclein, seeded with MSA aggregates
By onRaw data associated with DOI: 10.1038/s41467-021-22108-0
Visualisation and quantification of dendritic spines in cultured human Medium Spiny Neurons (MSNs)
By onThis protocol describes the visualisation of dendritic spines of human neurons cultured on coverslips in vitro and subsequent quantification using the software Imaris.
RAB3 phosphorylation by pathogenic LRRK2 impairs trafficking of synaptic vesicle precursors
By onThe authors’ results reveal a mechanism by which pathogenic hyperactive LRRK2 may contribute to the altered synaptic homeostasis associated with characteristic non-motor and cognitive manifestations of PD.
Intracellular Cytokine (ICS) Staining Protocol
By onThis protocol details about intracellular cytokine (ICS) staining.
Immunofluorescent labeling of cultured cells
By onThis protocol describes the process of Paraformaldehyde fixation and immunofluorescent-labeling of cultured cells.
Pluripotency markers staining
By onThis protocol describes the standard procedure for staining pluripotency markers, e.g., OCT4, SSEA4, alkaline phosphatase, and etc. on human pluripotent stem cells (hPSCs).
Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures–Fluorospot SFC
By onFluorospot SFC (combined IFNg, IL-5, and IL-10) data and corresponding HC-PD group IDs described in "Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures"
Immunofluorescence assay (IFA)
By onThis protocol details the procedure of immunofluorescence assay (IFA).
Raw tomogram of endogenous a-synuclein inclusion in primary mouse neurons seeded with PFFs
By onRaw data associated with DOI: 10.1038/s41467-021-22108-0
Cryo-ET of S. cerevisiae during starvation (tomograms)
By onCryo-ET of S. cerevisiae during starvation (tomograms).
Optogenetic experiments with iLID system
By onThis protocol details experiments with the iLID optogenetic system as performed to acutely recruit Miro to mitochondria.
Membrane interaction and mechanism of LC3 lipidation machinery in autophagy – Raw GUV data
By onRaw GUV data of fluorescent protein imaged on a Nikon A1 confocal microscope with a 63 × Plan Apochromat 1.4 NA objective. Three biological replicates were performed for each experimental condition. Identical laser power and gain settings used.
3D-correlative FIB-milling and Cryo-ET of Autophagic structures in Yeast Cells
By onThis protocol describes how to plunge-freeze yeast on EM grids and how to target autophagic structures by combining cryo confocal fluorescence data to FIB-milling and tomogram acquisition.
Expansion Microscopy
By onExpansion microscopy is a technique to visualize biological structures with higher spatial resolution than traditional microscopy methods.
Unconventional Initiation of PINK1/Parkin Mitophagy by Optineurin
By onCargo sequestration is a fundamental step of selective autophagy in which cells generate a double membrane structure termed an autophagosome on the surface of cargoes. The authors uncover an unconventional path of PINK1/Parkin mitophagy initiation.
