Protocol Archive

SOP for cDNA synthesis by RT (Reverse Transcription) Promega kit.

QuBit HS RNA quantification.

This protocol details how to purify recombinant very low-density lipoprotein receptor (VLDLR)-C-tag protein from HEK293E cells.

This protocol details how to efficiently make in vitro and isolate Clu-phospholipid particles using purified Clusterin from HEK293E cells (dx.doi.org/10.17504/protocols.io.bvvkn64w) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC).

This protocol details how to efficiently label a protein at the N-terminus using Clusterin protein as example.

This protocol details how to efficiently monitor Clusterin and Clusterin/Substrate uptake in different cell types like HEK293T, iNeurons, and iMicroglia.

The open field test is a classical test for the quantification of locomotor activity and anxiety-like behaviors in laboratory animals. This protocol is optimized for mice, and it includes the automatic analysis of their movement by Biobserve Viewer.

The parallel beam test protocol is designed to evaluate the motor coordination of mice. This protocol is designed to assess motor skills, particularly in neurological diseases such as Parkinson's disease.

This protocol describes the setup and execution of the light-dark box test for mice, a behavioral experiment used to assess anxiety-related responses. The protocol involves preparing a light-dark box in a controlled environment, utilizing specific lighting conditions and camera settings for optimal observation and recording.

The Rotarod test protocol for mice assesses motor coordination and balance. The test measures the latency to fall and speed at the fall.

The locomotion test is an open field test in darkness, so it does not aim to measure anxiety, only locomotor activity.

The Live Mouse Tracker (LMT) system is engineered for long-term, automated tracking and behavioral analysis of mice, integrating machine learning, computer vision, and RFID technology for simultaneous monitoring of multiple subjects.

The Fontana-Masson staining protocol is a detailed method for staining free-floating sections (20-50 microns) to detect melanin and other pigments in tissue samples.

The elevated plus maze is a classical test to screen anxiety-like behaviors in laboratory animals.

This protocol outlines a method for modulating noradrenergic and dopaminergic neuronal activity. Developed for longitudinal studies spanning up to four weeks, the protocol aims to assess neuromelanin's activity-dependent accumulation through daily agonist administration.

This protocol describes an optogenetic activation of neuromelanine-laden dopaminergic neurons in the SNc-VTA.

This protocol covers chronic optrode construction, implantation, and recording in the Locus coeruleus (LC).

This protocol details a Data Independent Acquisition (DIA)-based mass spectrometry (MS) data acquisition method for proteomic profiling of the Golgi, including a description of how to construct the nano Liquid chromatography and DIA MS methods and a Data Dependent Acquisition (DDA) strategy to generate deep spectral libraries for searching the DIA data.

Grip strength test for mice.

The protocol covers the steps to measure neuromelanin-positive neuron density in substantia nigra using image analysis tools, including NZConnect (Hamamatsu), a web-based whole-slide image (WSI) viewer, Cellpose, and QuPath.