Protocol Archive

This protocol details the purification and activation of Mouse Naïve CD8+ T Cell from mouse spleen.

This protocol outlines the maintenance, differentiation, and harvesting of the CLU198 embryonic mouse hippocampal cell line.

The V-PLEX panel offers analytically validated multiplex assay kits. Developed under rigorous design control, V-PLEX kits provide accurate and reproducible results with consistency from lot to lot. The V-PLEX Proinflammatory Panel 1 (human) Kit includes ten biomarkers that are associated with the inflammatory response and immune system regulation.

TEM protocol.

Bulk tissue RNA-sequencing of hiPSC-derived astrocytes, neurons, and co-cultures using the Illumina TruSeq Stranded mRNA Library Prep kit.

Protocol for running an SDS-PAGE.

Harvesting and performing single-cell RNA-seq.

Protein FTIR protocol.

This protocol describes the method to perform patch-clamp recordings of iPSC-derived neurons.

This protocol describes a method of generating aggregation of human recombinant alpha-synuclein.

Protocol for validating behavioral response to IP injection of clozapine-n-oxide in DAT-IRES-Cre mice injected with AAV-DIO-hM3Dq-DREADD in midbrain.

This protocol describes steps for ex vivo electrophysiology in mouse brain slices. This protocol also includes instructions for clozapine N-oxide (CNO) testing in DREADD-expressing neurons.

This protocol describes the steps for acquiring photometry data for freely moving mice.

A protocol for transfecting iPSC-derived midbrain dopaminergic neurons with antisense oligonucleotides (ASOs).

This protocol describes steps for immunofluorescent staining of free floating fixed mouse brain tissue sections.

This protocol describes steps for immunohistochemical 1 3,3’-Diaminobenzidine (DAB) staining of free floating fixed mouse brain tissue sections.

This protocol describes steps for optical density analysis of immunohistochemical 3,3′-Diaminobenzidine (DAB) staining of mouse brain sections using ImageJ.

This protocol details Hematoxylin and Eosin Stain for paraffin-embedded tissue sections.

This protocol describes a bioluminescence stepwise imaging protocol in mice with AAV-luciferase expression in the vagal afferents using the Ivis Lumina series III imaging system.

This protocol describes the behavioral test to measure food intake in mice.