Protocol Archive

This protocol describes how to install the second or third phase of the two-part chamber system in rhesus macaques, as well as the craniotomy. The first baseplate installation is described in a separate protocol titled, "Baseplate Implantation for Two-Part Chamber System in Rhesus Monkeys."

Protocol details implanting a baseplate onto the skull as the first step in creating a chamber system in rhesus macaques. The thin baseplate is enclosed subcutaneously to promote healing and osseointegration without infection risks.

This protocol describes the preparation of fly food with small molecules to treat flies during their aging. It can be adapted for any treatments. Details for small molecules used can be found in the "Materials" section.

This protocol describes the practical steps to perform behavioral monitoring using ethoscopes. The cleaning and preparation of glass tubes as well as loading the flies into ethoscopes is outlined.

This protocol allows to assess the susceptibility of flies to seizure-like behavior based on mechanically hyper-stimulating sensory inputs and assessing seizure-like activity.

This protocols describes the recording of electroretinograms (ERGs) and calculation of genetic interactions of fly mutants for a transheterozygous screen.

This protocol is used to visualize dopaminergic neurons in Drosophila brains, but can be adapted for other targets as well.

This protocol describes how to perform the Startle induced negative geotaxis (SING) assay. SING defects in Parkinsonism fly models have previously been shown to be caused by dopaminergic impairments.

Masson-fontana staining for melanin detection.

Optical densitometry of striatal neuronal fibers in the rodent brain.

Immunochemistry protocol on rodent brain cryosections.

Hematoxylin and Eosin (H&E) staining for histological sections.

Nissl Counterstaining with Cresyl Violet for histological sections.

This protocol details how to set up and run a direct ELISA on your protein of interest.

The protocol describes head-mounted miniscope calcium imaging data acquisition for NHPs.

Mice survival analysis.

Reproduction analysis in mice.

A viral vector was used in NHP to spread a mutated gene, inducing synucleinopathy in brain areas connected to the putamen. After four weeks, animals were studied to quantify affected neurons and their distribution.

This protocol describes how to express and purify mCherry-ATG101/13 (1-191aa) subcomplex.

This protocol describes how to express and purify mCherry- or GFP-tagged ATG13/101 subcomplex.