Introducing GolgiTag to Cells and Immunoprecipitation of Golgi
By Blythe Lloyd onA protocol for rapidly purifying Golgi across various cell lines and tissues without any modification. The Golgi purified using this method are highly enriched, intact, contaminant-free and, depending on solubilisation buffer, could be used for various downstream applications, such as proteomics and metabolomics. Access the interview with Rotimi Fasimoye, PhD, about this protocol.
Intracellular Cytokine (ICS) Staining Protocol
By Blythe Lloyd onThis protocol details about intracellular cytokine (ICS) staining.
Insect Cell Protocol for LRRK1 and LRRK2 Expression
By Blythe Lloyd onProtocol for expressing LRRK1 and LRRK2 in insect cells.
In Vivo Electrophysiology (Mouse)
By Blythe Lloyd onThis protocol describes the in vivo electrophysiology method for recording neuronal activity in mice.
Immunohistochemistry
By Blythe Lloyd onThis protocol describes immunohistochemical staining of fixed brain sections.
Imaris Coloc Protocol
By Blythe Lloyd onImaris intensity-based protocol for Colocalization analysis.
GUV assay
By Blythe Lloyd onThis protocol describes the preparation of GUVs for the reconstitution of LC3 lipidation reaction through the steps of PI3P production by PI3KC3-C1, WIPI2 recruitement and LC3 lipidation with PE. Screen reader support enabled.
GST Bead pulldown Assay
By Blythe Lloyd onGST Pulldown Assay for recruitment of bait proteins to GST labeled prey proteins. Prey proteins can be purified or from lysate.
Glucosylceramidase Beta (GBA) Genotyping
By Blythe Lloyd onThis protocol has been adapted from the PRoBaND Clinical Consortium (incorporating methods described by Neuman et al., 2009 and Stone et al., 2000) and has been used for all publications for PRoBaND / Tracking Parkinson's describing clinical data and outcomes with respect to GBA status.
Fiber Photometry (Mouse)
By Blythe Lloyd onThis protocol describes the procedure for fiber photometry in awake behaving mice. It includes details on the surgical implantation of fibers.
Expression and purification Twin-STREP-FLAG tagged ATG13:ATG101 constructs
By Blythe Lloyd onExpression and purification Twin-STREP-FLAG tagged ATG13:ATG101 constructs
Expression and purification protocol of WIPI2d
By Blythe Lloyd onThis protocol details the expression and purification protocol of WIPI2d.
Expression and purification protocol of GST-TAX1BP1
By Blythe Lloyd onThis protocol details the expression and purification protocol of GST-TAX1BP1.
Expression and purification protocol of GST-mCh-FYVE
By Blythe Lloyd onThis protocol details the expression and purification of GST-mCh-FYVE.
Expression and purification MBP-ATG9 Constructs
By Blythe Lloyd onExpression and purification from HEK cells of ATG13, ATG101 and FOLDON-ATG9A proteins.
Expression and purification GST-tagged ATG13:ATG101 constructs
By Blythe Lloyd onExpression and purification of GST-tagged ATG13/ATG101 constructs.
Ex Vivo Electrophysiology
By Blythe Lloyd onThis protocol describes preparation of brain slices, setup of electrophysiology rig, and solutions for collecting whole cell and cell attached recordings.