Protocol Archive

This protocol is part of a collection of protocols (dx.doi.org/10.17504/protocols.io.8epv593dng1b/v1) for the paper, "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function" (https://doi.org/10.21203/rs.3.rs-1521848/v1).

A procedure for quantitative real time reverse transcription PCR of α-synuclein, TNF, and NF-κβ.

Soluble/insoluble alpha-synuclein fractionation is a technique used to separate different forms of the alpha-synuclein protein based on their solubility properties.

Cell culture of STC-1 mouse enteroendocrine cells.

This protocol outlines all procedures for handling of tissue of animal subjects treated with AAV.

This protocol outlines procedures to perform immunohistochemistry on tissue that had been treated with AAV.

This protocol outlines procedures to extract viral DNA and whole RNA from rhesus macaque tissue that had been treated with AAV in vivo.

This protocol describes the dissection and collection of coronal sections of the striatum and midbrain from a mouse brain.

This protocol describes two behavioral tasks for mice. The first is the Open Field Test, which is used to asses motor behavior, and the second is the T-Maze, which is used to assess spatial learning.

This protocol describes live imaging of primary neuron cultures. Included are methods for preparing hippocampal or dopamine neuronal cultures from neonatal mouse brain tissue. The imaging technique was used in Jain et al., 2023 to compare vesicular release in neurons between various transgenic knockout mouse lines.

This protocol describes immunohistochemical and immunocytochemical methods to analyze protein expression in mouse brain. In the protocol, we describe the fixation of tissue or cultured neurons, the immunostaining procedure, and collecting images for analysis.

This protocol describes the method of Fast Scan Cyclic Voltammetry (FSCV) for the application of measuring dopamine transients in mouse brain slices. Within the protocol are sections describing the manufacture of microelectrodes, preparation of acute brain slices, fiber calibration, and measuring stimulated dopamine release.

This is a collection of protocols associated to the manuscript "Characterizing the diversity of enteric neurons using Dopamine Transporter (DAT)-Cre reporter mice."

This protocol provides the details for how to transfect and harvest 3X-FLAG-SHIP164Δ901-1099.

This protocol details siRNA-mediated knockdown of TFE3.

This protocol describes the RNA isolation from cultured cells and quantitative RT PCR.

Lentiviral production protocol.

This protocol describes how to detect alpha-synuclein in protein derived from STC-1 cells by western blot using DAB/peroxidase or ECL to visualize the bands.

When the cells undergo another stimulation (through KCL or electrical), the FM1-43 dye containing vesicles will be released through exocytosis, thus, resulting in a decrease in the FM1-43 fluorescence intensity.

This protocol is about nuclei isolation and sorting from frozen human temporal cortex.