Protocol Archive

This protocol is used to measure Transferrin uptake as a way to investigate Clathrin-mediated endocytosis in HIPCS derived neurons, or other cell types.

Fluorescence in situ hybridization (FISH) in Conserved and cell type-specific transcriptional responses to IFN-γ in the ventral midbrain.

Immunohistochemistry for Conserved and cell type-specific transcriptional responses to IFN-γ in the ventral midbrain.

Stereotaxic injections for Conserved and cell type-specific transcriptional responses to IFN-γ in the ventral midbrain.

This protocol describes cell surface biotinylation.

This protocol describes cellular lipid uptake with flow cytometry readout.

This protocol describes immunofluorescent staining with anti-GFP and anti-CD63 antibodies.

This protocol describes the regular maintenance and passaging human iPSCs.

This protocol describes microsomal membrane isolation from cell culture.

This protocol describes western blotting.

This protocol describes Chu Lab's standard external solutions for electrophysiology.

This protocol describes brain slice preparation for electrophysiology recording.

This protocol describes immunohistochemistry.

This protocol describes confocal imaging on a Nikon A1R confocal microscope and digital image analysis.

This protocol describes the cylinder behavior test.

This protocol describes the open-field locomotion test.

This protocol describes the quantification of TH immunoreactivity.

This protocol gives a step-by-step guide to imaging oligomers in human brain tissue using a spinning disk confocal microscope. The microscope used for this protocol was a commercial set up produced by 3i; however the techniques described can be used as general guidelines with alternative microscopes.

This protocol describes quantifying the LAMP1 positive puncta.

This protocol details how to purify 3xFLAG-SHIP164Δ901-1099.