Biochemical detection of aggregated Tau
By quincy.tichenor onThe AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds.
Mouse Stereotaxic Surgery
By Michelle onThis protocol describes the steps for performing stereotaxic surgery in mice. It is applicable to intracranial injections (e.g. virus, drug) and placement of implants (e.g. optical fibers, electrode arrays) into targeted regions of mouse brains.
Optogenetic Manipulation (Mouse)
By Michelle onThis protocol describes the steps for in vivo ontogenetic manipulation in mice, including assembly of fiber-ferrules, surgical implantation of fibers, and testing procedure.
Plunge freezing
By Michelle onThis protocol describes the procedure of plunge freezing for subsequent cryo-electron tomography.
Post mortem human substantial nigra TH staining
By Michelle onThis protocol is standardized for postmortem (frozen) SN tissue from pathologically diagnosed PD patients and control individuals for Immunofluorescence staining.
Proteomics workflow for whole cell lysate, endosome, and lysosome fractions
By Michelle onA protocol for sample preparation for LC-MS analysis of whole cell lysates and for lysosomal and endosomal fractions purified by Lyso-IP and Endo-IP.
Purification and Crystallization of ATG9 HDIR-ATG101:ATG13 complex
By Michelle onPurification and Crystallization of ATG9 HDIR (828-839) fused ATG101 (1-198):ATG13 (1-197).
Purification of the PE2 nCas9-RT protein
By Michelle onThis protocol describes the process of expressing and purifying the nicking Cas9-MMLV RT fusion protein for prime editing.
Purifying Vps13-VAB domain proteins
By Michelle onIn this protocol, we have listed the steps to purify the Vps13 adaptor binding domain (or VAB) with or without PxP motif fusion peptide derived from adaptor proteins Mcp1, Ypt35 or Spo71.
Rab8a expression and purification
By Michelle onRecombinant Rab8a expression and purification protocol as used by the Leschziner and Reck-Peterson Labs.
Reconstitution of LRRK2 membrane recruitment onto planar lipid bilayers
By Michelle onA method to monitor the recruitment of purified LRRK2 kinase onto planar lipid bilayers containing lipid-anchored Rab10 protein using Total Internal Reflection Fluorescence (TIRF) Microscopy.
Midbrain-like Organoids generation from hiPSCs
By Blythe Lloyd onProtocol for generating midbrain organoids from human iPSCs. In this protocol we describe the differentiation of human induced pluripotent stem cells (hiPSCs) into human midbrain-like organoids (hMLOs). This protocol has been developed based from several published protocols.
LRRK2RCKW Widefield fluorescence microtubule binding assay
By Blythe Lloyd onThis assay uses TMR labeled LRRK2 or LRRK1 RCKW to measure binding to microtubules in vitro
LRRK2 microtubule sedimentation binding assay
By Blythe Lloyd onAssay to determine LRRK2 protein binding to microtubules.
LRRK2 Immunofluorescent staining
By Blythe Lloyd onProtocol for immunofluorescent staining for LRRK2 in cultured cells using the MJFF2 (c41-2) antibody.
Investigation_of_mitophagy_in_Hippo_neurons
By Blythe Lloyd onWe developed a method for assessing mitochondrial clearance in primary hippocampal neurons.