Immunofluorescence of macrophages and microglia
By onThis protocol describes the fixation and staining of cultured cells in paraformaldehyde solution.
Standard Operating Procedure: Mouse transcardiac perfusion protocol
By onMouse transcardiac perfusion protocol and storage.
Preparing samples for NGS
By onThis protocol describes a standard procedure used to prepare PCR samples for Next Generation Sequencing (NGS).
Sample Collection and Processing for RNA Analysis
By onThis protocol delineates the steps taken to collect frozen postmortem human brain samples and process them for RNA extraction and analysis.
Isolation of NeuN+ cells from brain tissue (for CUT and RUN)
By onThis protocol describes the steps to isolate NeuN+ cells from brain tissue in preparation for CUT and RUN.
Sample preparation for TMT-based total and phospho-proteomic analysis of cells and tissues
By onMass spectrometry-based proteomics and phosphoproteomics are highly sensitive and unbiased techniques to study the proteome and phosphoproteome at a global scale.
Manual isolation of nuclei from human brain using CellRaft device and single nucleus Whole Genome Amplification
By onProtocol for manual nuclear isolation from human brain tissue using Cell Raft device for single cell Whole Genome Amplification
Purifying Vps13-VAB domain proteins
By onIn this protocol, we have listed the steps to purify the Vps13 adaptor binding domain (or VAB) with or without PxP motif fusion peptide derived from adaptor proteins Mcp1, Ypt35 or Spo71.
Standard Operating Procedure: Vibratomes
By onSOP outlines safe usage of vibratomes for cutting tissue samples.
Sample Collection and Measurement of Serum Neurofilament Light (NfL)
By onThis protocol details the steps for the preparation of serum from human blood samples and the measurement of NfL concentration using the NF-Light Advantage kit on the HD-X Analyzer (Quanterix, Billerica, MA).
Soluble and insoluble A-SYN fractionation
By onSoluble/insoluble alpha-synuclein fractionation is a technique used to separate different forms of the alpha-synuclein protein based on their solubility properties.
Preparing whole cell samples for immunoblot analysis
By onProtocol for preparation of HeLa cell lysates for immunoblot analysis.
Preparation of soluble and insoluble mitochondrial protein fractions for immunoblotting
By onProtocol describing the preparation procedure of soluble and insoluble mitochondrial protein fractions from HeLa cells for immunoblotting.
Mitochondrial isolation from HeLa cells
By onMitochondrial isolation and quantification from HeLa cells.
Reverse-phase high pH fractionation (using Thermo Fisher, Cat# 84868)
By onThis protocol uses the Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher, Cat# 84868)
Preparation of soluble and insoluble mitochondrial protein fractions for mass spectrometry analysis
By onProtocol describing preparation of soluble and insoluble mitochondrial protein fractions from HeLa cells for mass spectrometry analysis.
Measuring mitophagy via FACS with mtKeima reporter
By onPreparation of samples for measuring mitophagy levels using mtKeima reporter by fluorescence activated cell sorting (FACS).
qRT-PCR sample preparation
By onProtocol for qRT-PCR sample preparation for analysis using a RotorGeneQ machine (Qiagen).
