Preparation of soluble and insoluble mitochondrial protein fractions for immunoblotting
By onProtocol describing the preparation procedure of soluble and insoluble mitochondrial protein fractions from HeLa cells for immunoblotting.
Mitochondrial isolation from HeLa cells
By onMitochondrial isolation and quantification from HeLa cells.
Preparation of soluble and insoluble mitochondrial protein fractions for mass spectrometry analysis
By onProtocol describing preparation of soluble and insoluble mitochondrial protein fractions from HeLa cells for mass spectrometry analysis.
Reverse-phase high pH fractionation (using Thermo Fisher, Cat# 84868)
By onThis protocol uses the Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher, Cat# 84868)
Measuring mitophagy via FACS with mtKeima reporter
By onPreparation of samples for measuring mitophagy levels using mtKeima reporter by fluorescence activated cell sorting (FACS).
qRT-PCR sample preparation
By onProtocol for qRT-PCR sample preparation for analysis using a RotorGeneQ machine (Qiagen).
Salivary collection participant instructions using OMNIgene ORAL OM 501 device
By onSalivary collection participant instructions using OMNIgene ORAL OM 501 device
Medium fractionation and EV preparation
By onThis protocol describes the characterization of the extracellular alpha-synuclein and DNAJC5
Cryo-EM sample preparation for PI3KC3-C1~RAB1A complex
By onThis protocol details cryo-EM sample preparation for PI3KC3-C1~RAB1A complex.
Electrospray ionization analysis of eluted nucleotides
By onThis protocol describes the denaturation of PI3KC3-C1, and subsequent analysis of eluted nucleotides by electrospray ionization mass spec.
Preparing mitochondrial samples for immunoblot analysis
By onProtocol for preparation of mitochondrial samples for immunoblot analysis.
Protein Digestion and Mass Spectrometry Analysis Protocol
By onThis protocol details the procedure for the trypsin digestion of PI3KC3-C1 and subsequent analysis of digested peptides using LC-MS/MS.
Preparation of tissue for Ribo-Tag/RNAseq analysis
By onThis protocols describes the RiboTag virus vectors injected into mouse brain, tissue dissection, immunoprecipitation and RNA extraction for RNAseq.
Section 1: Enzymatic DNA Fragmentation (Manually)
By onThis protocol details manual enzymatic DNA Fragmentation prior to Section 2: NGS library preparation for sequencing.
Section 2: NGS library preparation for sequencing
By onThis protocol details NGS library preparation for sequencing and should be performed after Section 1: Enzymatic DNA Fragmentation (Manually).
Targeted detection of SNCA CNVs in SOX10+ nuclei from oligodendrocytes containing alpha-synuclein inclusions isolated from human post-mortem brain
By onThis protocol describes the detection of low-level genomic mosaics in the human brain using DNA immunofluorescence-fluorescence in situ hybridisation, known as immuno-FISH, to analyze copy number variants in the SNCA gene related to PD & MSA.
Preparation of fresh paraformaldehyde for mouse perfusion and tissue fixation
By onPreparation of fresh paraformaldehyde for mouse perfusion and tissue fixation.
Making Carbon-Fibre Microelectrode (CFM) for electrochemical recordings of monoamines in ex vivo mouse brain slices
By onThis protocol is to make carbon-fiber microelectrode (CFM) for electrochemical recordings of monoamines in ex-vivo mouse brain slices.
