Preparation of Holding ACSF (artificial cerebrospinal fluid) for slice electrophysiology
By onPreparation of Holding ACSF Holding ACSF is used for incubating brain slices prior to cell-attached or whole-cell electrophysiological recordings of neurons.
Preparation of Recording ACSF (artificial cerebrospinal fluid) for slice electrophysiology
By onPreparation of Recording ACSF Recording ACSF is used for incubating brain slices during cell-attached or whole-cell electrophysiological recordings of neurons.
Isolation of lysosomes using the Tagless LysoIP method in PBMCs
By onDetailed protocol for rapid isolation of intact lysosomes (including from clinical samples) for subsequent -omics analyses.
Sample preparation protocol for proteomic analysis of isolated lysosomes and whole cell extracts
By onHere, the authors describe a step-wise protocol for proteomic sample preparation derived from cell line models or isolated human cells. The protocol has been optimized for organelle pulldown preps.
Mouse tissue collection
By onThis protocol is about collecting mouse tissues for immunohistochemistry or biochemistry experiments. While it is mainly used for brain tissues, it also can be used for other visceral tissues.
Mouse sample collection for metabolomics studies
By onProtocol used in the Mazmanian lab for collecting brain and gut tissues and plasma from mouse for metabolomics.
A method for RNA extraction, cDNA synthesis and and quantitative PCR from NIH-3T3 fibroblasts
By onProtocol for RNA extraction, cDNA synthesis, and qPCR from NIH-3T3 fibroblasts outlined. Method applicable to mouse embryonic fibroblasts as well.
DNA extraction protocol for saliva samples obtained from Omigene oral OM device using the Qiagen QiaAMP Powerfecal DNA kit for shotgun metagenomic sequencing
By onThis protocol describes the procedure for DNA extraction of saliva samples using the Omnigene OM device and the Qiagen QiaMP Powerfecal DNA PRO kit for shotgun metagenomic sequencing.
Lysosomal isolation protocol
By onProtocol for isolating lysosomes from cultured cells is described.
Immunofluorescent Labeling of Preformed Fibril Protein Protocol
By onProtocol outlines use of AlexaFluor 488 kit to label alpha synuclein preformed fibrils for fluorescence, facilitating future applications.
Fecal Sample Collection
By onFecal sample collection from mice to use for gut microbiome sequencing analysis.
Protocol for ventriculography-assisted stereotaxic surgery in non-human primates
By onProtocol for the intraparenchymal delivery of AAVs in the NHP brain by taking advantage of stereotaxic surgery assisted by intrasurgical ventriculography
Protocol for non-human primate necropsy
By onA step-by-step guide explaining how to extract the brain and the spinal cord in non-human primates
Rodent brain processing for histological analyses
By onProtocol for rat brain processing in order to perform histological analyses
