Fecal Sample Collection
By onFecal sample collection from mice to use for gut microbiome sequencing analysis.
Protocol for non-human primate necropsy
By onA step-by-step guide explaining how to extract the brain and the spinal cord in non-human primates
Protocol for ventriculography-assisted stereotaxic surgery in non-human primates
By onProtocol for the intraparenchymal delivery of AAVs in the NHP brain by taking advantage of stereotaxic surgery assisted by intrasurgical ventriculography
Nissl Counterstaining – Cresyl Violet
By onNissl Counterstaining with Cresyl Violet for histological sections
Hematoxylin and Eosin (H&E) Staining
By onHematoxylin and Eosin (H&E) Staining for histological sections
Immunohistochemistry on free-floating cryosections
By onImmunochemistry protocol on rodent brain cryosections
Optical densitometry of neuronal fibers
By onOptical densitometry of striatal neuronal fibers in the rodent brain
Rodent brain processing for histological analyses
By onProtocol for rat brain processing in order to perform histological analyses
Brain Clarification for Neuromelanin visualisation by OPT
By onProtocol for the clarification of whole mouse brains to allow neuromelanin visualisation by Optical Projection Tomography
In situ Hybridization for Tyrosinase
By onIn situ hybridization protocol with DIG-labelled riboprobes
Human Brain Sequential Extraction (Tau)
By onHuman brain sequential detergent fractionation for PHF tau extraction
Bulk tissue RNA-sequencing – hiPSC-derived astrocytes, neurons and co-cultures
By onBulk tissue RNA-sequencing of hiPSC-derived astrocytes, neurons and co-cultures using the Illumina TruSeq Stranded mRNA Library Prep kit
Multifiber Array Fabrication and Calibration
By onThis protocol describes a multi-fiber photometry approach to track dopamine release in mice with high spatial and temporal resolution in multiple locations in the brain simultaneously.
Tissue and blood collection related to “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By onTissue and blood collection related to "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration"
Preparation of pharmacological agents
By onThis is a description of preparation of different drugs for use in live animals (in vivo) or in brain slices (ex vivo).
