Production of GTPase Deficient RAB1A(Q70L) Protein
By onBacterial expression of the GTPase deficient Q70L mutant of human RAB1A.
Preparation of pharmacological agents
By onThis is a description of preparation of different drugs for use in live animals (in vivo) or in brain slices (ex vivo).
Immunoblotting of i3 Neurons and dopaminergic neurons
By onThis protocol describes the preparation of cell lysate from and iPSC-derived neurons (I3 Neurons, dopaminergic) and the immunoblotting procedure.
Crude Subcellular fractionation of FAM177A1-GFP expressing cells
By onThis protocol details the crude subcellular fractionation of FAM177A1-GFP expressing cells.
Fluorescence size exclusion chromatography (FSEC) of native nanodiscs
By onThis is a protocol describing how to conduct Fluorescence size exclusion chromatography of native nanodiscs.
Making Ca2+ – selective microelectrodes
By onProtocol for making Ca2+ sensitive electrodes to measure extracellular Ca2+ concentrations at high spatiotemporal resolution in acute ex vivo brain slices.
Perfusion/ Fixation for cryostat slicing for HHC
By onThis protocol details the Perfusion and Fixation for cryostat slicing for HHC.
Systemic AAV administration through retro-orbital injection
By onProtocol for administering an AAV systemically through injection into the retro-orbital sinus of mouse
Absolute quantification of AAV genomes from total DNA with digital droplet PCR (ddPCR)
By onProtocol for absolute measurement of AAV genome number from total DNA samples extracted from animal tissues
Protocol for isolating mitochondria from mouse striatum
By onProtocol used in the Mazmanian lab to isolate striatal mitochondria from mouse for subsequent Seahorse and other analyses
Protocol for mouse striatal tissue dissection, mitochondrial protein extraction, and western blot analysis
By onProtocol used in the Mazmanian lab for preparation and analysis of mitochondrial proteins from the striatum of mouse.
RNA In situ hybridization (ISH) using RNAscope®
By onThis protocol outlines the procedures for RNA in situ hybridization to simultaneously detect up to three targets using the RNAscope® Multiplex Fluorescent v2 assay (ACD).
In Situ Hybridization -Mendelsohn et al 2025
By onThis protocol outlines the steps for preparing mouse brain tissue for In Situ Hybridization from Mendelsohn et al 2025.
Immunohistochemistry (Histology + Imaging)-Mendelsohn et al 2025
By onThe protocol outlines the steps for preparing mouse brain tissue for immunostaining and imaging from Mendelsohn et al 2025.
Histology Fushiki et al 2024
By onHistology protocol for Fushiki et al 2024 , to quantify Th+ cells and visualize the dorsal-tier or ventral-tier of SNc DANs.
Immunocytochemistry (Henderson Lab)
By onProtocol for immunocytochemistry on mammalian cells in 24 and 96 well plates.
Coronal cryosectioning of mouse brains
By onProtocol used in the Gradinaru lab for coronal cryosectioning of mouse brain tissue with a cryostat
