Optical densitometry of neuronal fibers
By onOptical densitometry of striatal neuronal fibers in the rodent brain
Hematoxylin and Eosin (H&E) Staining
By onHematoxylin and Eosin (H&E) Staining for histological sections
Immunohistochemistry on free-floating cryosections
By onImmunochemistry protocol on rodent brain cryosections
Nissl Counterstaining – Cresyl Violet
By onNissl Counterstaining with Cresyl Violet for histological sections
In situ Hybridization for Tyrosinase
By onIn situ hybridization protocol with DIG-labelled riboprobes
Brain Clarification for Neuromelanin visualisation by OPT
By onProtocol for the clarification of whole mouse brains to allow neuromelanin visualisation by Optical Projection Tomography
Human Brain Sequential Extraction (Tau)
By onHuman brain sequential detergent fractionation for PHF tau extraction
Bulk tissue RNA-sequencing – hiPSC-derived astrocytes, neurons and co-cultures
By onBulk tissue RNA-sequencing of hiPSC-derived astrocytes, neurons and co-cultures using the Illumina TruSeq Stranded mRNA Library Prep kit
Multifiber Array Fabrication and Calibration
By onThis protocol describes a multi-fiber photometry approach to track dopamine release in mice with high spatial and temporal resolution in multiple locations in the brain simultaneously.
Tissue and blood collection related to “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By onTissue and blood collection related to "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration"
Production of GTPase Deficient RAB1A(Q70L) Protein
By onBacterial expression of the GTPase deficient Q70L mutant of human RAB1A.
Preparation of pharmacological agents
By onThis is a description of preparation of different drugs for use in live animals (in vivo) or in brain slices (ex vivo).
Crude Subcellular fractionation of FAM177A1-GFP expressing cells
By onThis protocol details the crude subcellular fractionation of FAM177A1-GFP expressing cells.
Immunoblotting of i3 Neurons and dopaminergic neurons
By onThis protocol describes the preparation of cell lysate from and iPSC-derived neurons (I3 Neurons, dopaminergic) and the immunoblotting procedure.
Fluorescence size exclusion chromatography (FSEC) of native nanodiscs
By onThis is a protocol describing how to conduct Fluorescence size exclusion chromatography of native nanodiscs.
Making Ca2+ – selective microelectrodes
By onProtocol for making Ca2+ sensitive electrodes to measure extracellular Ca2+ concentrations at high spatiotemporal resolution in acute ex vivo brain slices.
Perfusion/ Fixation for cryostat slicing for HHC
By onThis protocol details the Perfusion and Fixation for cryostat slicing for HHC.
