Coronal cryosectioning of mouse brains
By onProtocol used in the Gradinaru lab for coronal cryosectioning of mouse brain tissue with a cryostat
Sample Preparation for Proteomic Analysis of Isolated Mitochondria and Whole-Cell Extracts
By onProtocol outlines precise sample prep for mass spectrometry-based proteomics, emphasizing robust lysis, S-trap column protein capture, and optimized digestion for high-quality data on proteomic changes and post-translational modifications.
AAV Production Protocol-Arber Lab
By onAAV Production methods used in Falasconi & Kanodia et al 2025 and is the general protocol for Arber Lab.
Preparation of midbrain slices for electrophysiology
By onProtocol for the preparation of midbrain slices for electrophysiology
Preparation of tissue punches of SNpc for the analyses of mitochondrial function
By onProtocol for the preparation of tissue punches of SNpc for the analyses of mitochondrial function
Immunohistochemistry & Microscopy-Falasconi & Kanodia et al 2025
By onImmunohistochemistry and microscopy methods used in Falasconi & Kanodia et al 2025.
Solutions used for FISH Codetection
By onProtocol to make the solutions used in the protocol published by AWM lab for FISH Codetection
MS sample prep Quantitative whole cell proteomics
By onDescribes sample preparation procedure for Quantitative Whole Cell Proteome Analysis by Mass Spectrometry
Fabrication and calibration of multi-fiber arrays to monitor dopamine release in the mouse striatum
By onA new method allows monitoring dopamine release in mice with high spatial and temporal resolution in multiple locations in the striatum simultaneously using a fluorescent sensor. The protocol involves array fabrication and pre-implant mapping.
Perfusion and Histology Rodrigues-Vaz and Athalye et al, 2025
By onThis is the standard protocol for perfusion and histology of brain slices for Rodrigues-Vaz and Athalye et al, 2025.
Synaptic vesicle preparation from mouse brain
By onProtocol for isolating synaptic vesicles from mouse brains. Resulting SV-enriched fractions are used for biochemical analyses.
Mass spectrometry analysis with Data Independent Acquisition (DIA)
By onDetailed protocol for DIA-based MS data acquisition method is provided here.
Rapid Isolation of Lysosomes (LysoIP) from mouse brain tissue
By onProtocol for rapid isolation of lysosomes from mouse brain tissue using LysoIP developed to assess lysosomal protein profiles. Enables detailed proteomic analysis of lysosomes, critical for biomolecule degradation and recycling.
Benzoyl-Hexose derivatization
By onHexose derivatization boosts hexose sugar detection in samples for LC-MS analysis.
BioID: Identifying Protein-Protein Interactions in Living Cells
By onBioID method uses BirA* to biotinylate proteins near a target protein in cells. This allows isolation of biotinylated proteins for identification using mass spectrometry.
Sample preparation for proteomic analysis of LysoIP
By onSample preparation in proteomics is vital for accurate data acquisition and analysis using techniques like LC-MS. Methods range from in-gel digestion to in-solution prep, all following similar principles and steps.
