Synaptic vesicle preparation from mouse brain

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Description

This protocol describes the preparation and purification of synaptic vesicles (SVs) from the brains of mice. Brain tissue is homogenized in sucrose-based SHT buffer, followed by sequential centrifugation steps to obtain the LP2 fraction. Synaptic vesicles are subsequently purified by glycerol gradient centrifugation, and SV-enriched fractions are collected for downstream biochemical analyses.

Identifier (DOI)

10.17504/protocols.io.j8nlkyqndg5r/v1

Tags

Organelle

Discovery Team

Team Edwards

Program

Collaborative Research Network

Theme

Circuitry and Brain-Body Interactions

Meet the Authors

Akio Mori

External Collaborator
Robert Edwards, MD

Lead PI (Core Leadership): Team Edwards,

University of California, San Francisco

Synaptic vesicle preparation from mouse brain

Output Details

Meet the Authors

Akio Mori

Robert Edwards, MD

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