Sample preparation for proteomic analysis of LysoIP

In a proteomics pipeline a correct sample preparation is a crucial step as it deeply influence the subsequent data acquisition (usually performed by liquid chromatography couple to mass spectrometry LC-MS) as well as data analysis. This procedure can be conducted using different techniques, from in-gel digestion to filter based sample preparation. The present document illustrate how to perform in-solution sample preparation. Regardless of which procedure is chosen, all sample preparation techniques share the same basic principles and follows similar step-wise procedure (Muntel et al., 2019; Ombrato et al., 2019; Sacramento et al., 2019).