Crystallization, data collection and structure solution of PxP-VAB
By onProtocol lists the steps to obtain PxP(Mcp1ct)-VABct crystals, X-ray diffraction data acquisition and obtaining the structure solution using molecular replacement with a model generated by AlphaFold2.
Preparing plasmids for nucleofection of hPSCs
By onThis protocol describes the standard procedure for the preparation of plasmids to be delivered into human pluripotent stem cells (hPSCs) using nucleofection.
Cell lysis and immunoblotting for protein and phospho-protein quantification
By onThe authors describe the procedure by which human iPSC-derived neurons or mouse embryonic fibroblasts (MEFs) were lysed and probed for levels of proteins of interest using Western blot.
Tissue handling for mice, marmoset, and rhesus macaques injected with AAV
By onThis protocol outlines all procedures for handling of tissue of animal subjects treated with AAV.
Neural differentiation on EM grids – iNeurons sample preparation for cryo-ET and CLEM
By onProtocol for direct differentiation of AAVS1-TRE3G-NGN2 iPSCs and hESCs to iNeurons on EM grids: coat grids, seed iNeurons, transduce with fluorescent proteins, then plunge freeze for cryo-ET and cryo-CLEM.
Cryo-EM sample preparation
By onThis protocol details how to prepare cryo-EM samples of 3xFLAG-SHIP164Δ901-1099
Striatal dopamine measurement through HPLC
By onProtocol for striatal dopamine measurement in mouse brain
Immunofluorescence of macrophages and microglia
By onThis protocol describes the fixation and staining of cultured cells in paraformaldehyde solution.
Standard Operating Procedure: Mouse transcardiac perfusion protocol
By onMouse transcardiac perfusion protocol and storage.
Preparing samples for NGS
By onThis protocol describes a standard procedure used to prepare PCR samples for Next Generation Sequencing (NGS).
Sample Collection and Processing for RNA Analysis
By onThis protocol delineates the steps taken to collect frozen postmortem human brain samples and process them for RNA extraction and analysis.
Isolation of NeuN+ cells from brain tissue (for CUT and RUN)
By onThis protocol describes the steps to isolate NeuN+ cells from brain tissue in preparation for CUT and RUN.
Sample preparation for TMT-based total and phospho-proteomic analysis of cells and tissues
By onMass spectrometry-based proteomics and phosphoproteomics are highly sensitive and unbiased techniques to study the proteome and phosphoproteome at a global scale.
Manual isolation of nuclei from human brain using CellRaft device and single nucleus Whole Genome Amplification
By onProtocol for manual nuclear isolation from human brain tissue using Cell Raft device for single cell Whole Genome Amplification
Purifying Vps13-VAB domain proteins
By onIn this protocol, we have listed the steps to purify the Vps13 adaptor binding domain (or VAB) with or without PxP motif fusion peptide derived from adaptor proteins Mcp1, Ypt35 or Spo71.
Standard Operating Procedure: Vibratomes
By onSOP outlines safe usage of vibratomes for cutting tissue samples.
Sample Collection and Measurement of Serum Neurofilament Light (NfL)
By onThis protocol details the steps for the preparation of serum from human blood samples and the measurement of NfL concentration using the NF-Light Advantage kit on the HD-X Analyzer (Quanterix, Billerica, MA).
Soluble and insoluble A-SYN fractionation
By onSoluble/insoluble alpha-synuclein fractionation is a technique used to separate different forms of the alpha-synuclein protein based on their solubility properties.
Preparing whole cell samples for immunoblot analysis
By onProtocol for preparation of HeLa cell lysates for immunoblot analysis.
