Protocol for dissection of midbrain from mice, followed by isolation of RNA from midbrain samples, reverse transcription of mRNA to cDNA, and quantitative real-time PCR for analysis of VMAT2 expression levels.
Protocol for RT-qPCR to test levels of NF-kB response genes in a cell model transiently over-expressing Parkin. We found upregulation of key pro-inflammatory genes normalized to housekeeping gene, Gapdh.
