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Lentivirus preparation using Lenti-X Concentrator

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Output Details

Description
This protocol describes the preparation of Lenti-GCaMP6 viral particles using a 2nd generation lentiviral system.  Briefly HEK293FT cells were transfected and Lentivial particles were collected 24 and 52h post-transfection. Lentiviral production success was confirmed using Lenti-X GoStix ( following manufacture's instructions). Lentiviral particles were concentrated using Lenti-X Concentrator (following manufacturer's instructions). 
Identifier (DOI)
10.17504/protocols.io.3byl4ddz2lo5/v1
Tags
  • Lentivirus
Discovery Team
Team Scherzer
Program
Collaborative Research Network
Theme
PD Functional Genomics

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