Electrophysiological validation of striatal neurons and dopamine neurons in D1-Cre, A2a-Cre, D1-FlpO, A2a-FlpO, DAT-FlpO transgenic mice
By onElectrophysiology dataset from whole-cell recordings from murine striatal spiny projection and midbrain dopaminergic neurons from acute slices prepared from knock-in transgenic mouse lines: D1-Cre- A2a-Cre- D1-FlpO- A2a-FlpO- DAT-FlpO.
Generation of knock-in Cre and FlpO mouse lines for precise targeting of striatal projection neurons and dopaminergic neurons
By onTo enable precise genetic targeting of key neuronal populations in the basal ganglia, we generated and characterized five knock-in mouse lines: Drd1-Cre, Adora2a-Cre, Drd1-FlpO, Adora2a-FlpO, and DAT-FlpO.
Dataset for Alcacer et al., Cell Reports 2025
By onData set from manuscript Abnormal hyperactivity of striatal ensembles encodes distinct dyskinetic behaviors revealed by high-resolution clustering, Alcacer et al., Cell Reports 2025.
Videos from Alcacer et al., Cell Reports 2025
By onVideo files from manuscript Abnormal hyperactivity of striatal ensembles encodes distinct dyskinetic behaviors revealed by high-resolution clustering, Alcacer et al., Cell Reports 2025.
Pipeline for Image Processing and Quantification of Striatal Dopamine Innervation using Fiji (IF/IHC)
By onThis protocol outlines the image preprocessing steps required prior to analysis with ImageJ tools as well as the ImageJ pipeline to quantify striatal innervation.
Calcium and Behavior Video Alignment Scripts for Open-Field Dyskinesia Analysis (Alcacer et al., Cell Reports 2025)
By onMatlab scripts for alignment of calcium imaging videos and videos from mice in an open field arena recorded from a bottom camera from Alcacer et al. 2025.
FISH CODETECTION
By onDIG and Fluorescein labeled riboprobes Fluorescent in situ hybridization Frozen brain sections
Solutions used for FISH Codetection
By onProtocol to make the solutions used in the protocol published by AWM lab for FISH Codetection
Determining the effects of mecamylamine in the mouse striatum using a Conditioned Preference Place (CPP) paradigm
By onProtocol tests mecamylamine effects in mouse striatum using CPP paradigm to measure preference for drug-associated environment.
Intracranial injections of viral vectors in mouse midbrain and striatum
By onProtocol for injecting viral vectors in mouse midbrain and striatum is provided.
Immunocytochemistry of acute brain slices used in ex vivo voltammetry recordings
By onProtocol labels ChAT for cholinergic interneurons and TH for dopaminergic neurons in mouse brain slices. Immunofluorescence used post ex vivo voltammetry recordings on 300-µm thick slices.
In vivo voltammetry and fiber photometry in the mouse striatum
By onProtocol measures dopamine and calcium levels in striatal dopaminergic axons in vivo via voltammetry and fiber photometry in response to electrical stimulation.
Wide-field imaging of voltage sensors expressed in ex vivo mouse brain slices
By onThis protocol describes how to perform wide-field imaging of voltage sensors using high frame rates (660 Hz minimum every 2.5 minutes) in mouse midbrain using ex vivo brain slices.
Imaging axonal calcium dynamics in ex vivo mouse brain slices
By onProtocol for imaging calcium dynamics in striatal dopaminergic axons in ex vivo mouse brain slices using GCaMP6f in DAT-Cre:Ai95D mice. Calcium transients were observed in response to single and train electrical stimuli.
Fast-scan cyclic voltammetry to assess dopamine release in ex vivo mouse brain slices following electrical or optogenetic stimulations
By onThis protocol is to assess changes in extracellular dopamine concentration following electrical or optogenetic stimulations in ex vivo mouse brain slices.
Datasets and Key Resources Table used in Zang, Y. et al. (2025) “An axonal brake on striatal dopamine output by cholinergic interneurons”
By onThis repository contains a 1) Key Resources Table with details on key resources and the persistent identifiers for protocols and code used and generated in this study; 2) Source Data Folder, and 3) Supplementary Data Folder.
Microinvasive Probes for Monitoring Electrical and Chemical Neural Activity in Nonhuman Primates
By onWe designed carbon fiber sensors that enable long-term simultaneous recording of both neurochemical and electrical neural activity in deep brain structures of nonhuman primates.
Custom MATLAB scripts related to Zhang, Y. et al (2025) “An axonal brake on striatal dopamine output by cholinergic interneurons”
By onMATLAB scripts written by Yan-Feng Zhang to predict how nicotinic receptors impact on dopamine transient in vivo during the dynamic tonic and multiphasic activity in cholinergic interneurons.
s-µIP Fabrication Process
By onThis protocol describes the fabrication of s-µIP microelectrodes. These devices are made of either one or two parylene-coated tungsten-carbon fiber assemblies, threaded through a silica tube.
