ASAP CRN Cloud Release Notes – Version 2.0.0
By onVersion: 2.0.0 Release Date: December 11, 2024 This release notes document provides a concise overview of the updates and enhancements introduced in Version 2.0.0 of the CRN Cloud platform.
Extracellular space diffusion modelling identifies distinct functional advantages of archetypical glutamatergic and GABAergic synapse geometries
By onWe modelled extracellular diffusion in super-resolved images of live brain tissue neuropil. We found that extracellular space geometry shapes local diffusion and this has functional implications for signaling arising from synaptic spill-over.
ASAP CRN Cloud Release Notes – Version 1.0.0
By onVersion: 1.0.0 Release Date: June 25, 2024 This release notes document provides a concise overview of the updates and enhancements introduced in Version 1.0.0 of the CRN Cloud platform.
ASAP CRN Cloud Release Notes – Version 1.0.0-beta
By onVersion: 1.0.0-beta Release Date: March 6, 2024 This release notes document provides a concise overview of the updates and enhancements introduced in Version 1.0.0-beta of the CRN Cloud platform.
ASAP CRN Cloud Release Notes – Version 0.0.1
By onVersion: 0.0.1 Release Date: November 10, 2023 This release notes document provides a concise overview of Version 0.0.1 of the CRN Cloud platform.
Release Notes – ASAP CRN Cloud
By onSummary: Release notes for version 3.0.0 of CRN Cloud platform, released on September 30, 2025, detailing latest updates and enhancements.
Quantification of Axonal Projections from Whole-Brain Slide Images
By onQuantification of Axonal Projections from Whole-Brain Slide Images provides a standardized method for processing and analyzing immunofluorescence images of mouse brain sections to quantify axonal projections.
IBA1 Immunochemistry on human paraffin sections
By onIba1-specific Immunochemistry protocol on paraffin-embedded human brain sections
Olympus Slideview VS200″ slide scanning
By onBrief guide on the main steps to follow in order to scann a sample with the "Olympus Slideview VS200 slide scanner. Although examples for certain parameters are presented, each user should choose them based on the characteristics of their samples.
Cytokine Array
By onThe EMD MILLIPLEX‱ MAP Human Cytokine/Chemokine/Growth Factor Pane A – Immunology Multiplex Assay (Merck Millipore) is employed to simultaneously analyse TNF-α, IL-1β, IL 6, and INFγ with Bead-Based Multiplex Assay using the Luminex‱ technology.
Gene expression analysis
By onTo evaluate whether human microglia cells (hMG) can be activated by neuromelanin (NM), hMG were cultured with and without the presence of NM for 24 h and then harvested to measure gene expression of selected pro-inflammatory and neurotoxic factors.
Immunocytochemistry (ICC) detection of key microglia markers in iPSC derived human microglia cells
By onImmunocytochemistry (ICC) detection of key microglia markers in iPSCderived human microglia cells (hMG)
Intracellular ROS measurement in iPSC-derived microglia
By onIntracellular ROS measurement in iPSC-derived microglia
