The human autophagy-initiating complexes ULK1C and PI3KC3-C1
By onULK1C and PI3KC3-C1 are crucial for macroautophagy initiation. Recent research focuses on their structural organization, activation mechanisms, and roles in autophagy initiation's protein interaction network for potential therapeutic targeting.
PET-CT related to “Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and parkinsonian motor deficits”
By onPET-CT related to "Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and parkinsonian motor deficits"
Generation of isogenic iPSC lines through CRISPR/Cas9-Mediated Targeting
By onProtocol for creating genetically modified human pluripotent stem cell lines using CRISPR/Cas9: Guide RNAs inserted into pX330 Cas9 plasmid, delivered with targeting vectors into hPSCs.
How Membrane Contact Sites Shape the Phagophore
By onPhagophores form membrane contact sites with organelles like the vacuole, ER, and lipid droplets. In situ imaging techniques, such as cryo-CLEM, provide valuable insights into these interactions, aiding in understanding autophagosome biogenesis.
pCDH1-CMV-FIP200-V44D-mCherry-Hygro
By onLentiviral expression vector encoding FIP200 mutant-mCherry
