Autophagy detection assay
By onAutophagosome detection using Dojindo DAPRed dye and Lysotracker Green in neurons
Immunoprecipitation from primary neurons
By onProtocol for immunoprecipitating proteins from neuronal lysates
Assay for lysosomal activity using Magic Red
By onAssay for lysosomal Cathepsin B activity in neurons using Magic Red
Mitophagy detection assay
By onDetection of mitophagy flux in neurons using Dojindo Mtphagy dye and Lysotracker Green
Data related to “Mitochondrial damage triggers the concerted degradation of negative regulators of neuronal autophagy”
By onData related to "Mitochondrial damage triggers the concerted degradation of negative regulators of neuronal autophagy"
A lipid transfer-dependent feedback loop activates ATG9A compartments in autophagy initiation
By onPhagophore formation requires ATG9, ATG2 and PI3KC3-C1. Authors show that ATG2 is crucial for PI3P appearance on ATG9A compartments with little PI, suggesting a lipid transfer-driven feedback loop supporting lipid transfer and ATG8 lipidation.
Derailed degradation: LRRK2-dependent exocytosis in Parkinson’s disease
By onFerguson discusses new data on LRRK2 mutations increasing neuronal exocytosis of vesicles enriched in endosomal components. LRRK2 may promote lysosome exocytosis through interactions with GABARAP and Rab GTPases.
BenPadman/AIVE: AIVE release 1_Basic scripts and macros for implementing AIVE: AI-directed Voxel Extraction.
By onBenPadman/AIVE: AIVE release 1
EMPIAR-12885 Datasets associated with AI-directed voxel extraction and volume EM identify intrusions as sites of mitochondrial contact
By onCollection of datasets used in "AI-directed voxel extraction and volume EM identify intrusions as sites of mitochondrial contact"
Microwave assisted sample processing with cultured cells for 3D FIB-SEM
By onRoutine procedure for microwave assisted sample processing of biological tisses for 3D FIB-SEM.
Key Resouce Table (KRT) & Numerical data used in “AI-directed voxel extraction and volume EM identify intrusions as sites of mitochondrial contact”
By onKey Resource Table (KRT) detailing quantitative data, code, protocols, and key lab materials used in "AI-directed voxel extraction and volume EM identify intrusions as sites of mitochondrial contact".
3D distance measurements & rendering with AIVE results
By onProcedure for conducting 3D distance measurements and visualizations (with Paraview and Blender Respectively)
Processing 3D FIB-SEM data via AIVE
By onA complete procedure for processing 3D FIB-SEM data via AIVE (AI-directed Voxel Extraction); from raw images to final result.
Structure of the human prohibitin complex in the closed state
By onStructure of the human prohibitin complex in the closed state
3D spatial alignment of multiple similar objects extracted from FIB-SEM data using Fijiyama
By onProcedure for the use of Fijiyama ("Yet Another Matching and Alignment tool for Fiji") to spatially align a series of similar cellular structures detected from 3D FIB-SEM data.
Preparation of 3D distance probes for the quantification of variability in distance measurements between conditions
By onSimple procedure for preparing random 3D probe points for the analysis of image segmentation variability
Structure of the human prohibitin complex in the open state_EMDB-70180
By onStructure of the human prohibitin complex in the open state
