Figure 1: Rubicon KO Starvation and Mitophagy HaloTag processing assay
By onFigure 1. Rubicon depletion promotes bulk autophagy and Parkin mitophagy.
Figure 4: The Pacer RH domain basic triad controls subcellular localization [FINAL]
By onFigure 4. The Pacer RH domain basic triad controls subcellular localization.
Figure 3: Rubicon – Rab7 binding colocalization [FINAL] Creators
By onFigure 3. pRAB7A binding preferences dictate subcellular localization.
Figure 7. Pacer expression promotes formation of WIPI2 puncta upon depolarization [FINAL]
By onFigure 7. Pacer expression promotes the formation of WIPI2 puncta upon depolarization.
Figure 6: Pacer LC3B imaging [FINAL]
By onFigure 6. pRAB7A-dependent function of Pacer in mitophagosome expansion.
Figure S3: Immunofluorescence of mitochondria alongside LC3B [FINAL]
By onSource Data for Figure S3 in https://doi.org/10.1083/jcb.202309015.
Figure S2. pS72 Rab7 formation following depolarization, starvation, and iron chelation [FINAL]
By onFigure S2. pS72 Rab7 formation following depolarization, starvation, and iron chelation.
Embryonic/Postnatal Mouse Neuron Culture Protocol
By onThis protocol details the primary neuron culture procedures from embryonic and postnatal mice.
Gradient-index (GRIN) lens implantation surgery in non-human primates
By onProcedures involve injecting AAV-GCaMP6 into the SMA of non-human primates for calcium imaging experiments. A GRIN lens is then inserted into the injected area, protected by a custom chamber with a lid for reuse.
FSCV and Ephys Recording Setup
By onMethods detailing the recording setup using both electrophysiological (Ephys) and fast-scan cyclic voltammetry (FSCV) used by Amjad et al. 2024 10.1523/ENEURO.0001-24.2024.
Preparation of human iPSC-derived cortical neuronal progenitors for transplantation into the rodent brain
By onThis protocol describes how we prepare human iPSC-derived cells, that have been differentiated into cortical neuronal progenitors, for transplantation into the brain of immunocompromised athymic mice. Neuronal progenitors mature within the mouse brain and are used to study the pathogenesis of Parkinson’s disease.
Code for extracting FSCV spikes
By onCode for extracting FSCV spikes used in Amjad et al. 2024 10.1523/ENEURO.0001-24.2024.
Code for simulating FSCV artifacts
By onCode for simulating FSCV artifacts for algorithm validation in Amjad et al. 2024 10.1523/ENEURO.0001-24.2024
Isolation of lysosomes using the Tagless LysoIP method in PBMCs
By onDetailed protocol for rapid isolation of intact lysosomes (including from clinical samples) for subsequent -omics analyses.
Generation of stable LysoTag expressing cell lines and LysoTag immunoprecipitation of lysosomes
By onProtocol for generating LysoTag (TMEM192-3xHA) cells and immunoprecipitation of lysosomes for subsequent -omics analyses.
In vivo Electrophysiology Protocol
By onProtocol outlines method for collecting extracellular single unit dopamine recordings.
