Expression and purification protocol of WIPI2d
By Blythe Lloyd onThis protocol details the expression and purification protocol of WIPI2d.
Expression and purification protocol of GST-TAX1BP1
By Blythe Lloyd onThis protocol details the expression and purification protocol of GST-TAX1BP1.
Expression and purification protocol of GST-mCh-FYVE
By Blythe Lloyd onThis protocol details the expression and purification of GST-mCh-FYVE.
Expression and purification MBP-ATG9 Constructs
By Blythe Lloyd onExpression and purification from HEK cells of ATG13, ATG101 and FOLDON-ATG9A proteins.
Expression and purification GST-tagged ATG13:ATG101 constructs
By Blythe Lloyd onExpression and purification of GST-tagged ATG13/ATG101 constructs.
Ex Vivo Electrophysiology
By Blythe Lloyd onThis protocol describes preparation of brain slices, setup of electrophysiology rig, and solutions for collecting whole cell and cell attached recordings.
LRRK2 kinase activity regulates GCase level and enzymatic activity differently depending on cell type in Parkinson’s disease
By taliag onPublished: Recent work suggests that LRRK2 kinase activity can modulate glucocerebrosidase (GCase) function. The authors investigated the relationship between LRRK2 and GBA1 by assessing GCase activity and found a positive correlation between the activities of LRRK2 and GCase in different cellular and ex vivo models. View original preprint.
Electron microscope sample preparation technique_V2
By Blythe Lloyd onThere are eight main steps in preparing EM sample, including fixation, en bloc staining, agarose embedding, dehydration, infiltration, resin embedding, resin-embedded sample trimming and ultrathin sectioning. This EM sample preparation protocol is designed for processing cultured cell sample.
Design and preparation of synthetic reference peptides for APP/Aβ TOMAHAQ proteomics, version 2
By Blythe Lloyd onThis protocol describes the design and preparation of synthetic reference peptides for APP/Aβ TOMAHAQ proteomics.
Deep Brain Stimulation (DBS) Implant
By Blythe Lloyd onThis protocol describes the steps for making implants used for deep brain stimulation (DBS) in rodents.
Crystallization, data collection and structure solution of PxP-VAB
By Blythe Lloyd onProtocol lists the steps to obtain PxP(Mcp1ct)-VABct crystals, X-ray diffraction data acquisition and obtaining the structure solution using molecular replacement with a model generated by AlphaFold2.
Sample Collection and Measurement of Serum Neurofilament Light (NfL)
By Michelle onThis protocol details the steps for the preparation of serum from human blood samples and the measurement of NfL concentration using the NF-Light Advantage kit on the HD-X Analyzer (Quanterix, Billerica, MA).
Single-cell dissociation of brain organoids
By Michelle onSingle-cell dissociation of brain organoids
SNP Genotyping and ApoE Genotyping
By Michelle onThis protocol details the steps for DNA extraction from a human blood sample, quality control, and SNP and APOE genotyping. The protocol has been adapted from the PRoBaND SNP Genotyping and ApoE Genotyping Protocol
In situ architecture of the lipid transport protein VPS13C at ER-lysosomes membrane contacts
By Blythe Lloyd onPublication: Loss-of-function mutations in VPS13C are responsible for rare cases of familial early onset Parkinson’s disease. Using cryo-ET, the authors provide in-situ evidence for a bridge-model of VPS13 in lipid transport. View the original preprint.
DOPA pheomelanin is increased in nigral neuromelanin of Parkinson’s disease
By Blythe Lloyd onPublished: Neuromelanin in the substantia nigra may be a key factor contributing to dopaminergic neuron vulnerability in Parkinson’s disease. Neuromelanin consists of pheomelanin and eumelanin moieties. Here, authors investigated the relative composition and specific roles of pheomelanin and eumelanin moieties of NM in PD. View original preprint.
Staining of cells with GolgiTracker for Golgi flow cytometry analysis
By Michelle onMethod that allows the staining of intact Golgi using GolgiTracker for subsequent flow cytometry analysis. The analysis can provide a quantitative indication of the level of enrichment of the organelle immunoprecipitationand can be used as a quality control check for the purity of the immunoprecipitated Golgi.
Standard Operating Procedure: Vibratomes
By Michelle onThis Standard Operating Procedure (SOP) describes the safe use of vibratomes.
