Stable cell line generation via retrovirus
By onProduction of pseudotyped virus an subsequent transduction.
Isothermal Titration Calorimetry of the Rubicon RH domain and Rab7
By on1:1 binding of Rubicon and Rab7.
Homology Modelling of Pacer RH domain in SWISS-MODEL
By onUsing the Rubicon RH - Rab7 crystal structure as a template (6wcw).
Immunofluorescent Imaging and Analysis
By onFollowing treatment with antibodies/genetic methods of introducing fluorescent tags.
Expression and Purification of RH proteins via MBP affinity tag
By onAffinity purification followed by size exclusion chromatography.
Autophagosome live cell imaging
By onAutophagosome live cell imaging for the quantification of PINK-PARKIN mitophagy.
Plasmid construction – A RAB7A Phosphoswitch Coordinates Rubicon Homology Protein Regulation of Parkin- Dependent Mitophagy
By onPlasmid construction via restriction enzyme digest and ligation.
Figure 2. Rubicon and Pacer RH domain have inverted pRAB7A binding specificity [FINAL]
By onFigure 2. Rubicon and Pacer RH domain have inverted pRAB7A binding specificity.
