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WIPI2d puncta formation assay

By Dan Tudorica, BSc
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Output Details

Description
Protocol for WIPI2d puncta formation assay that labels sites of autophagosome generation.
Identifier (DOI)
10.17504/protocols.io.n2bvj3myxlk5/v1
Tags
  • Fluorescence imaging
  • Human
  • Microscopy - optical
Team
Team Hurley
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

  • Dan Tudorica, BSc

    Key Personnel: Team Hurley, 

    University of California, Berkeley

Related Research

Datasets and Key Resources Table used in Do, Quyen B. et al. (2023) “Early striatal hyperexcitability in an in vitro human striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation”

The files found here are associated to Do, Q. et al (2023) study and contain: (a) all source and supplementary data, (b) Key Resources Table, and (c) list of primers.

View Dataset

Live Imaging of Primary Mouse Neuron Cultures

This protocol describes live imaging of primary neuron cultures. Included are methods for preparing hippocampal or dopamine neuronal cultures from neonatal mouse brain tissue. The imaging described involves labeling of dopamine neurons with a…

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Immunohistochemistry

This protocol describes immunohistochemical staining of fixed brain sections.

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Aligning Science Across Parkinson's
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