Figure S4: MW weight of gels [FINAL]

Figure S4. MW verification of HaloTag flux reporters. (A) Cells stably expressing Parkin and Su9 HaloTag reporter were labelled with fluorescent halo ligand, and then were depolarized with OA for 2 hours as indicated in prior experiments. Lysates were run alongside an unstained protein ladder. After acquisition of fluorescent scans, the gel was stained with Coomassie blue dye and imaged to show molecular weight standards. (B) Cells stably expressing LC3-Halo were labelled with fluorescent Halo ligand, and then were starved for ~1 H as in prior experiments. A pre-stained molecular weight standard was diluted 1:10, and then 2 μL was run alongside the lysates from this experiment. (C) A western blot was performed against Pacer and Tubulin in Pacer KO and mCherry-Pacer expressing cells as in figure 5C. (D) Western blot against WT and Rubicon KO cells with indicated molecular weight markers.