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3D-correlative FIB-milling and Cryo-ET of Autophagic structures in Yeast Cells
Output Details
Description
This protocol describes how to plunge-freeze yeast on EM grids and how to target autophagic structures by combining cryo confocal fluorescence data to FIB-milling and tomogram acquisition.
Identifier (DOI)
10.17504/protocols.io.b79wrr7e