3D-correlative FIB-milling and Cryo-ET of Autophagic structures in Yeast Cells

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Description

This protocol describes how to plunge-freeze yeast on EM grids and how to target autophagic structures by combining cryo confocal fluorescence data to FIB-milling and tomogram acquisition.

Identifier (DOI)

10.17504/protocols.io.b79wrr7e

Tags

Autophagy
Cryo-ET

Discovery Team

Team Harper

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Cristina Capitanio

External Collaborator
Anna Bieber

External Collaborator
Philipp S. Erdmann

External Collaborator
Brenda Schulman, PhD

Co-PI (Core Leadership): Team Harper,

Max Planck Institute of Biochemistry
Florian Wilfling

External Collaborator
Wolfgang Baumeister

External Collaborator

3D-correlative FIB-milling and Cryo-ET of Autophagic structures in Yeast Cells

Output Details

Meet the Authors

Cristina Capitanio

Anna Bieber

Philipp S. Erdmann

Brenda Schulman, PhD

Florian Wilfling

Wolfgang Baumeister

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