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ASAP CRN Cloud Release Notes – Version 2.0.0
onVersion: 2.0.0 Release Date: December 11, 2024 This release notes document provides a concise overview of the updates and enhancements introduced in Version 2.0.0 of the CRN Cloud platform.
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ASAP CRN Cloud Release Notes – Version 1.0.0
onVersion: 1.0.0 Release Date: June 25, 2024 This release notes document provides a concise overview of the updates and enhancements introduced in Version 1.0.0 of the CRN Cloud platform.
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ASAP CRN Cloud Release Notes – Version 1.0.0-beta
onVersion: 1.0.0-beta Release Date: March 6, 2024 This release notes document provides a concise overview of the updates and enhancements introduced in Version 1.0.0-beta of the CRN Cloud platform.
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ASAP CRN Cloud Release Notes – Version 0.0.1
onVersion: 0.0.1 Release Date: November 10, 2023 This release notes document provides a concise overview of Version 0.0.1 of the CRN Cloud platform.
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Dephosphorylation of phosphorylated Rab GTPases by PPM phosphatases
onProtocol measures phosphoRab GTPase dephosphorylation with PPM phosphatases using purified phosphoRab GTPases. Substrates generated by phosphorylating Rab GTPases with MST3 kinase are included.
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Flowcytometry analysis of lysosomal pulldown with anti-TMEM192 magnetic beads from PBMCs
onMethod described for staining intact lysosomes with lysotracker for flow cytometry. Analysis provides quantitative indication of organelle enrichment in immunoprecipitation, serving as quality control.
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Sample Preparation for Proteomic Analysis of Isolated Mitochondria and Whole-Cell Extracts
onProtocol outlines precise sample prep for mass spectrometry-based proteomics, emphasizing robust lysis, S-trap column protein capture, and optimized digestion for high-quality data on proteomic changes and post-translational modifications.
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Mito-IP: Immunopurification of Mitochondria from MitoTag Cultured Cells
onA novel method called 'Mito-IP' enables rapid and pure mitochondrial isolation in 10 minutes, supporting various analyses. This technique is being extended to isolate other organelles like lysosomes, Golgi, and peroxisomes using epitope tags.
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GCase assay – Resorufin β-D-glucopyranoside
onMethod presented for assessing lysosomal enzyme GBA1 activity using Resorufin substrate in low pH conditions to minimize non-lysosomal activity. Validated in GBA1 KO cells, compatible with various lysates and adjustable for different GBA1 levels.
