-
Electrophysiological validation of striatal neurons and dopamine neurons in D1-Cre, A2a-Cre, D1-FlpO, A2a-FlpO, DAT-FlpO transgenic mice
onElectrophysiology dataset from whole-cell recordings from murine striatal spiny projection and midbrain dopaminergic neurons from acute slices prepared from knock-in transgenic mouse lines: D1-Cre- A2a-Cre- D1-FlpO- A2a-FlpO- DAT-FlpO.
-
Generation of knock-in Cre and FlpO mouse lines for precise targeting of striatal projection neurons and dopaminergic neurons
onTo enable precise genetic targeting of key neuronal populations in the basal ganglia, we generated and characterized five knock-in mouse lines: Drd1-Cre, Adora2a-Cre, Drd1-FlpO, Adora2a-FlpO, and DAT-FlpO.
-
The human autophagy-initiating complexes ULK1C and PI3KC3-C1
onULK1C and PI3KC3-C1 are crucial for macroautophagy initiation. Recent research focuses on their structural organization, activation mechanisms, and roles in autophagy initiation's protein interaction network for potential therapeutic targeting.
-
Dataset for Alcacer et al., Cell Reports 2025
onData set from manuscript Abnormal hyperactivity of striatal ensembles encodes distinct dyskinetic behaviors revealed by high-resolution clustering, Alcacer et al., Cell Reports 2025.
-
Videos from Alcacer et al., Cell Reports 2025
onVideo files from manuscript Abnormal hyperactivity of striatal ensembles encodes distinct dyskinetic behaviors revealed by high-resolution clustering, Alcacer et al., Cell Reports 2025.
-
PET-CT related to “Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and parkinsonian motor deficits”
onPET-CT related to "Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and parkinsonian motor deficits"
-
Generation of isogenic iPSC lines through CRISPR/Cas9-Mediated Targeting
onProtocol for creating genetically modified human pluripotent stem cell lines using CRISPR/Cas9: Guide RNAs inserted into pX330 Cas9 plasmid, delivered with targeting vectors into hPSCs.
-
Pipeline for Image Processing and Quantification of Striatal Dopamine Innervation using Fiji (IF/IHC)
onThis protocol outlines the image preprocessing steps required prior to analysis with ImageJ tools as well as the ImageJ pipeline to quantify striatal innervation.
-
Calcium and Behavior Video Alignment Scripts for Open-Field Dyskinesia Analysis (Alcacer et al., Cell Reports 2025)
onMatlab scripts for alignment of calcium imaging videos and videos from mice in an open field arena recorded from a bottom camera from Alcacer et al. 2025.
-
FISH CODETECTION
onDIG and Fluorescein labeled riboprobes Fluorescent in situ hybridization Frozen brain sections
