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HyDrop Bead Generation & PCR Barcoding V.2
onProtocol for producing dissolvable barcoded hydrogel beads used in HyDrop experiments.
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Structural remodeling of the mitochondrial protein biogenesis machinery under proteostatic stress
onCryo-ET showed protein aggregates, altered cristae, and reduced ribosome complexes in stressed mitochondria. Mitochondrial Hsp60 undergoes conformational changes to aid in protein folding, shedding light on mitochondrial proteostasis mechanisms.
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FISH CODETECTION
onDIG and Fluorescein labeled riboprobes Fluorescent in situ hybridization Frozen brain sections
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ATP13A4 gates extracellular polyamine levels to control excitatory synaptogenesis
onPolyamines are crucial for brain function. ATP13A4 is the main polyamine transporter in astrocytes, impacting astrocyte morphology, synapse formation, and neurodevelopment. Mutations in ATP13A4 are linked to neurodevelopmental disorders.
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Open Field Behavior in Mice-Ding Lab
onThe open field test is widely used to assess spontaneous locomotor activity, exploratory behavior, and anxiety-like responses in rodents.
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Single-cell extracellular recordings (surgery, STN optotagging, LHb recordings)
onProtocol for single cell extracellular recordings (surgery, STN optotagging, LHb recordings)
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Electrophysiological Recordings
onProtocol for electrophysiological patch clamp/voltage clamp recordings.
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Tabular datasets for “A role for the subthalamic nucleus in aversive learning”
onTabular datasets for "A role for the subthalamic nucleus in aversive learning." Includes FISH, electrophysiological, and behavioral datasets.
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Isolation of mouse midbrain, RNA purification, reverse transcription, and quantitative PCR
onProtocol for dissection of midbrain from mice, followed by isolation of RNA from midbrain samples, reverse transcription of mRNA to cDNA, and quantitative real-time PCR for analysis of VMAT2 expression levels.
