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A computational pipeline to quantify perinuclear lysosomes in fibroblasts using CellProfiler

By Ebsy Jaimon, Suzanne Pfeffer, PhD
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Output Details

Description
Here we present a CellProfiler software pipeline to quantify the distribution of lysosomes in MEF cells. The lysosomes were stained using anti-LAMP1 antibody, and nuclei were labeled using DAPI. The images were acquired using a Zeiss laser scanning confocal microscope and were maximum intensity projected in FIJI.
Identifier (DOI)
10.17504/protocols.io.81wgbxrw3lpk/v1
Discovery Team
Team Alessi
Program
Collaborative Research Network
Theme
PD Functional Genomics

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Aligning Science Across Parkinson's
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