A549 PIP4P1 (TMEM55B) KO (CVCL_D3VW)

By Prosenjit Pal, PhD

Output Details

Description
A549 PIP4P1 (TMEM55B) CRISPR/CAS9 A549 knock-out cell line generated from parental A549 cells (ATCC A549 CCl-185) using CRISPR/Cas9 gene-editing technology. The cell line was verified by immunoblotting analysis and DNA-sequencing analysis. Used in publication: doi: https://doi.org/10.1101/2023.06.07.544051
Tags
  • LRRK2 pathway
  • Rab
Team
Team Alessi
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

  • Prosenjit Pal, PhD

    Key Personnel: Team Alessi, 

    University of Dundee

Related Research

Parkinson’s VPS35[D620N] mutation induces LRRK2-mediated lysosomal association of RILPL1 and TMEM55B

This study uncovers a pathway through which dysfunctional lysosomes resulting from the VPS35[D620N] mutation recruit and activate LRRK2 on the lysosomal surface, driving assembly of the RILPL1-TMEM55B complex.

View Article

Primary data associated with the manuscript “Genome wide screen reveals Rab12 GTPase as a critical activator of pathogenic LRRK2 kinase” (doi: 10.1101/2023.02.17.529028)

Primary data associated with the manuscript "Genome wide screen reveals Rab12 GTPase as a critical activator of pathogenic LRRK2 kinase"

View Dataset

Primary data associated with the manuscript “Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55B” (doi: 10.1101/2023.06.07.544051)

Confocal microscopy images, CellProfiler Excel files of Pearson's coefficients between TMEM55B/RILPL1 or pRab10/RILPL1 in R1441C MEF or VPS35[D620N] MEF cells as seen in doi: 10.1101/2023.06.07.544051.

View Dataset
View More Outputs