Adapting hPSCs cultured on MEFs to feeder-free system

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Description

This protocol describes the procedure of adapting human pluripotent stem cells (hPSCs) to feeder-free culturing conditions using mTeSR-plus or StemFlex

Identifier (DOI)

10.17504/protocols.io.b4k5quy6

Tags

Feeder-free
hPSCs (Human pluripotent stem cells)

Discovery Team

Team Rio

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Frank Soldner, MD

Co-PI (Core Leadership): Team Rio,

Albert Einstein College of Medicine
Dirk Hockemeyer, PhD

Co-PI (Core Leadership): Team Rio,

University of California, Berkeley
Hanqin Li

External Collaborator
Oriol Busquets Figueras

External Collaborator
Steven Poser

External Collaborator

Adapting hPSCs cultured on MEFs to feeder-free system

Output Details

Meet the Authors

Frank Soldner, MD

Dirk Hockemeyer, PhD

Hanqin Li

Oriol Busquets Figueras

Steven Poser

Related Research

Feeder-free culturing of hPSCs

Passaging of feeder-free hPSCs

Preparing feeder-free hPSCs for nucleofection